China Plant leaf Direct PCR plus kit-UNG (ntle le Sampling Tools) Protocol Direct PCR from Plant Material Manufacturer and Supplier |Foregene

Lekhasi la semela Direct PCR hammoho le kit-UNG (ntle le lisebelisoa tsa sampole) Protocol Direct PCR ho tsoa ho Plant Material.

Tlhaloso ea Kit:

Ka ho toba sebelisa polysaccharide le polyphenol lekhasi lysate e le template bakeng sa PCR amplification, ntle le ho ntšoa ka thōko ho DNA, ka potlako le ka kutloisiso e phahameng.Lenaneo la UNG le khahlanong le tšilafalo le ekelitsoe ho thibela mekhoa e fosahetseng.

◮Ha ho tlhoekiso ea DNA e jang nako le e turang (Nako, mosebetsi le litšenyehelo li bolokiloe!)

◮Boitsebiso bo fokolang

◮E bonolo - sampole e ka ba tlas'a karabelo ea PCR kapa karabelo ea lysis ntle le ho seha kapa ho sila

◮2 X Mix-ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi

◮Tokiso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa ka metsotso e 50

◮High throughput-lysis reaction e ka phethoa ka poleiti ea PCR ea seliba tse 96

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Litlhaloso

Ho ipapisitsoe le Plant Leaf Direct PCR Plus kit, dUTP e sebelisoa sebakeng sa dTTP, 'me enzyme ea UNG e ka theolang thempleite e nang le dUTP ea eketsoa.Ka tsela ena, ho netefatsoa le ho nepahala ha ho holisa, 'me bothata ba tšilafalo ea sehlahisoa sa PCR bo ka hlahang nakong ea tlhahlobo e kholo ea liphatsa tsa lefutso bo thibeloa.

Litlhaloso

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Likarolo tsa Kit

Karolo ea I	Buffer P1
	Buffer P2
	6×DNA Loading Buffer
Karolo ea II	2× Lekhasi la PCR le bonolo^TMMotsoako(UNG)

Likarolo le melemo

■ Ha ho tlhoekiso ea DNA e jang nako le e theko e boima
■ Boitsebiso bo fokolang
■ E bonolo-sampole e ka ba tlas'a PCR reaction kapa lysis reaction ntle le ho seha kapa ho sila
■ 2 × Mix, ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi
■ Tokisetso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa bonyane ka metsotso e 50.
■ Boitšoaro bo phahameng ba throughput-lysis bo ka phethoa ka poleiti ea PCR ea seliba sa 96

Litekanyetso tsa kit

Kopo: boitsebiso bo fetotsoeng ka liphatsa tsa lefutso, genotyping, joalo-joalo.
Mohlala: lekhasi la semela
Tekanyo: Diameter 2-3mm (mokhoa o tobileng), bophara ba 5-7mm (mokhoa oa lysis)
Sebaka sa ho lemoha: sekhechana sa sepheo ≤1kb