Gel Extraction Kit DNA Gel Extraction Kit
Tlhaloso ea Kit:
Ka potlako le ka bokhabane fumana likhechana tsa DNA tsa 20bp-10kb ho jeleng ea agarose.
◮Mefuta e mengata ea ho hlaphoheloa ha DNA:Likaroloana tsa DNA tse khutšoane joalo ka 30bp le tse kholo joalo ka 10kb li ka fumanoa.
◮Bokhoni bo phahameng ba ho hlaphoheloa:Ts'ebetso e phahameng ka ho fetisisa ea ho hlaphoheloa e ka fihla ho feta 80%.
◮Khatiso e nyane ea sistimi:bonyane 30μl elution tharollo ka sebelisoa bakeng sa elution, e leng ka ka katleho eketsa mahloriso ea a hlaphoheloa likhechana tsa DNA.
◮Lebelo le potlakileng:Ho bonolo ho sebetsa, ho hlaphoheloa ha sekhechana sa DNA ho ka phethoa nakong ea metsotso e 15.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:Likaroloana tse fumanoeng tsa DNA li hloekile haholo, tse ka kopanang le liteko tse fapaneng tse latelang.
Tlhaloso
Setsi sena se sebelisa "spin column le formula" e ntlafalitsoeng ke k'hamphani ea rona, e ka khutlisang ka nepo likhechana tsa DNA tse hloekileng ho tloha jeleng ea agarose.Kit e ka khutlisa likhechana tsa DNA ka mefuta e mengata.Tlas'a maemo a tloaelehileng, likaroloana tsa DNA tsa 30bp-10kb li ka fumanoa.Bonyane 30μl ea leseli e ka sebelisoa ho eketsa bongata ba DNA e fumanoeng.
Sesebelisoa se bonolo ho sebetsa mme se na le mehato e fokolang.E hloka feela centrifugation e 'maloa ho sebetsana le lisampole tse ngata ka nako e le' ngoe, 'me likaroloana tsa DNA tse fumanoeng ka bohloeki bo phahameng li ka fumanoa ka metsotso e 15.
Litlhaloso
50 Preps
Buffer GE: Hlakola boloko ba gel ho 60°C ho lokolla likhechana tsa DNA ho tloha gel.
Buffer WB1: Tlosa li-ion tse setseng tsa letsoai ho DNA.
Buffer EB: hlahisa DNA ka har'a lera la tlhoekiso.
DNA-Feela Kholomo: Phatlalatso e khethehileng ea likhechana tsa DNA ka har'a gel.
Likarolo tsa Kit
| Buffer GE* |
| Sekhahla sa WB1 |
| Buffer EB |
| DNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
- Mefuta e mengata ea ho hlaphoheloa ha DNA: Likaroloana tsa DNA tse khutšoanyane joaloka 30bp le tse kholo joaloka 10kb li ka fumanoa.
-Bokhoni bo phahameng ba ho hlaphoheloa: Ts'ebetso e phahameng ka ho fetisisa ea ho hlaphoheloa e ka fihla ho feta 80%.
-Small system elution: bonyane 30μl elution solution e ka sebelisoa bakeng sa elution, e ka eketsang ka katleho mahloriso a likhechana tsa DNA tse fumanoeng.
-Lebelo le potlakileng: Ho bonolo ho sebetsa, ho hlaphoheloa ha sekhechana sa DNA ho ka phethoa nakong ea metsotso e 15.
-Tshireletso: Ha ho hlokahale ho ntšoa ha reagent ea organic.
-Boleng bo phahameng: Likaroloana tse fumanoeng tsa DNA li hloekile haholo, tse ka kopanang le liteko tse fapaneng tse latelang.
Sesebelisoa sa Kit
Setha sena se loketse ho khutlisa likhechana tsa DNA (30bp-10kb) ka lijelle tsa agarose.
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
Sekoti sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C), haeba se hloka ho bolokoa nako e telele, se ka bolokoa ho 2-8 ° C.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.
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