Metsi DNA Isolation Kit DNA Extraction le Purification Kit bakeng sa Metsi
Tlhaloso ea Kit:
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa ho likokoana-hloko ka lisampole tse fapaneng tsa metsi.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
Litlhaloso
50 Preps
-Lysozyme: enzymatic hydrolyze lerako la sele ea libaktheria tse ntle.
-Buffer TE: e sebelisetsoa ho lokisetsa tharollo ea 100mg / ml Lysozyme le ho fana ka tikoloho ea lysozyme enzymatic hydrolysis.
-Buffer SG1 & Buffer SG2: Fana ka tikoloho ea tšilo ea protease ea sampole.
-Forene Protease: Enzymatic hydrolyze disampole tikolohong ea protease enzymatic ho lokolla DNA ea genomic.
-Buffer SG3: Etsa hore Foregene Protease e sebetse 'me u fane ka tikoloho ea ho kenya DNA.
-Buffer SG4: Tlatsetso ea ho fana ka tikoloho ea ho kenya DNA.
-Buffer PW: Tlosa litšila tse kang protheine le RNA ho DNA.
-Buffer WB: Tlosa li-ion tse setseng tsa letsoai ho DNA.
-Buffer EB: hlahisa DNA ka har'a lera la tlhoekiso.
-DNA-feela Column: ka ho khetheha adsorb DNA genomic ka lysate.
Likarolo tsa Kit
| Buffer SG1 |
| Buffer SG2 |
| Buffer SG3 |
| Buffer SG4 |
| Sekhahla sa PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Foregene Protease |
| Lysozyme |
| DNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
-Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le RNase e eketsehileng nakong ea teko, ho qoba laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
-Lebelo le potlakileng: Ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša metsi ea genomic DNA e ka phethoa nakong ea metsotso ea 40.
-E bonolo: The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlaase oa centrifugation kapa ethanol precipitation ea DNA.
-Tshireletso: ha ho hlokahale ho ntšoa ha organic reagent.
-Boleng bo phahameng: Likaroloana tse nkiloeng tsa genomic DNA li kholo, ha ho na RNA, ha ho RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse fapa-fapaneng.
Sesebelisoa sa Kit
E loketse ho hloekisoa ha DNA ea genomic ea mehlala e latelang: metsi a letamo la aquaculture, metsi a letangoana, metsi a pompo, metsi a letša, metsi a nōka, metsi a letamo la lotus le mehlala e meng.
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
-Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C), haeba se hloka ho bolokoa nako e telele, se ka bolokoa ho 2-8 ° C.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o kenya tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e futhumatsa pele ka likhato tse 37°C ho hlapa metsi bakeng sa metsotso e 10 ho qhala metsi, 'me u kopanye pele o sebelisoa.
-Forene Protease tharollo e na le mokhoa o ikhethang, o sebetsang ha o bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);ha e bolokiloe ho 4 ℃, mosebetsi oa eona le botsitso li tla ba betere, kahoo ho kgothaletswa ho e boloka ho 4 ℃, hopola hore u se ke ua e boloka ho -20 ℃.
-Dry powder Lysozyme e bolokiloe ho -20 ° C;tharollo ea Lysozyme e lokiselitsoeng e arotsoe ka likaroloana tse nyenyane ebe e bolokoa ho -20 ° C.
