RT-qPCR Easyᵀᴹ (Mohato o le Mong) -SYBR Green I
Tlhaloso ea Kit:
◮Kit ea mohato o le mong e etsa transcript le qPCR karabelo e 'meli ka har'a tube e le' ngoe, e hloka feela ho eketsa template ea RNA, li-primer tsa PCR tse ikhethileng le RNase-Free ddH.2O.
◮Thepa e ka hlahloba RNA ea vaerase kapele le ka mokhoa o nepahetseng kapa ea lata RNA.
◮Setsi sena se sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
◮Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
◮RT-qPCR e bonoloTM(Mohato o le mong) -SYBR Green I kit e tla le dae ea ka hare ea ROX, e ka sebelisetsoang ho tlosa semelo sa matšoao le liphoso tsa matšoao lipakeng tsa liliba, tse loketseng bareki ho li sebelisa mefuteng e fapaneng ea lisebelisoa tsa PCR tse ngata.
Litlhaloso
RT-qPCR e bonoloTM (Mohato o le Mong) -SYBR Green I e sebelisa mokhoa o ikhethang oa ho kopanya ka katleho karabelo ea RT le ea Real Time PCR, e ka phethelang kapele tlhahlobo ea bongata ba liphatsa tsa lefutso.Sehlahisoa se na le mamello e matla, e khethehileng e phahameng le botsitso.Sehlahisoa sena se na le Foregene HotStar Taq DNA Polymerase e ikhethang ea k'hamphani, e nang le melemo ea ho holisa ka potlako (2Kb/min), bokhoni bo phahameng ba ho holisa, bokhoni bo khethehileng ba ho holisa, le sekhahla se tlase sa ho se lumellane ha se bapisoa le li-enzyme tse tloaelehileng tsa Taq.E sebelisoa mona bakeng sa Nako ea 'Nete RT-PCR ho fokotsa matlafatso e sa tobang le ho ntlafatsa ho nepahala ha PCR.
Litlhaloso
100 T (20μl tsamaiso)
500 T (20μl sistimi)
Likarolo tsa Kit
| RT-qPCR e bonoloTM(Mohato o le Mong) -SYBR Green I | ||
| Litaba tsa Kit | RT-02111 | RT-02112 |
| 100T (20μl sistimi) | 500T (20μl sistimi) | |
| 2 × RT-qPCR e BonoloTM Kopanya-SYBR | 1ml | 1.7ml×3 |
| 50 × ROX Reference Dye | 200μl | 1ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml×3 |
| Buka ea Tataiso | 1 sengoathoana | 1 sengoathoana |
Likarolo le melemo
- Kit ea mohato o le mong e etsa hore ho fetoleloe ka morao le qPCR ka makhetlo a mabeli ka har'a tube e le 'ngoe, e hloka feela ho eketsa template ea RNA, li-primer tsa PCR tse khethehileng le RNase-Free ddH.2O.
- Thepa e ka hlahloba RNA ea vaerase kapele le ka mokhoa o nepahetseng kapa ea ts'oara RNA.
- Thepa e sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
- Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
- RT-qPCR e bonoloTM(Mohato o le mong) -SYBR Green I kit e tla le dae ea ka hare ea ROX, e ka sebelisetsoang ho tlosa semelo sa matšoao le liphoso tsa matšoao lipakeng tsa liliba, tse loketseng bareki ho li sebelisa mefuteng e fapaneng ea lisebelisoa tsa PCR tse ngata.
Mokhoa oa ho sebetsa
Maemo a polokelo
1.Lipalangoangmaemo
Ts'ebetso eohle ea lipalangoang tsa li-ice box tsa mocheso o tlase, ho netefatsa hore kit e boemong ba <4 ℃.
2.Polokelo maemo
Thepa e bolokiloe ho -20 ℃.Boloka sehlahisoa ka sehatsetsing sa mocheso sa kamehla ho-20 ℃ hang ka mor'a ho fumana.Haeba maemo a polokelo a loketse, sehlahisoae ke ke ea nyenyefatsa ts'ebetso efe kapa efe nakong ea selemo sa 1 sa ho sebetsa.
