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Litharollo Tse 'ne Tse Ka Sehloohong Bakeng sa Taolo ea Tšilafalo ea Sehlahisoa sa PCR
1: Kenya sebaka sa lisebelisoa tsa liteko ka nako Seta (NTC) taolo e mpe 'me u e phete hangata.Hang ha ho fumanoa hore ho na le tšilafalo ea sehlahisoa sa PCR ka laboratoring, khutlisetsa lisebelisoa tsohle tsa liteko ka nako.Tse kang: hlapolla hape 'me u lokise li-primers, nchafatsa ntlha ea pipette, E ...Bala haholoanyane -
Li-enzyme tse peli tsa RT-PCR tse sebetsang habeli
Traditional reverse transcriptases ha e khone ho mamella mocheso o phahameng (mocheso o nepahetseng bakeng sa ts'ebetso ea MMLV ke 37-50 ° C, 'me AMV ke 42-60 ° C).RNA e rarahaneng haholo ea kokoana-hloko e ke ke ea fetoleloa ka mokhoa o nepahetseng ho cDNA ka mocheso o tlase, e leng se bakang katleho ea ho lemoha Phokotso.Tra...Bala haholoanyane -
Nucleic acid isothermal amplification theknoloji
PCR ke thekenoloji e sebelisoang ka ho fetisisa ea nucleic acid amplification 'me e sebelisoa haholo ka lebaka la kutloisiso le ho hlaka ha eona.Leha ho le joalo, PCR e hloka phepelo ea mocheso e phetoang khafetsa 'me ha e khone ho tlosa meeli ea ho itšetleha ka lisebelisoa le lisebelisoa, e fokotsang ts'ebeliso ea eona ho tsa bongaka ...Bala haholoanyane -
Ente ea mRNA ke eng?
Ente ea mRNA ke eng Ente ea mRNA e fetisetsa RNA liseleng tsa 'mele ho hlahisa le ho hlahisa li-antigen tsa protheine ka mor'a liphetoho tse nepahetseng tsa in vitro, ka tsela eo e lebisa' mele ho hlahisa karabelo ea 'mele ea ho itšireletsa mafung khahlanong le antigen, ka tsela eo ...Bala haholoanyane -
Theknoloji ea PCR
PCR (polymerase chain reaction) ke e 'ngoe ea litheknoloji tsa in-vitro DNA amplification, e nang le nalane ea lilemo tse fetang 30.Theknoloji ea PCR e ile ea qalisoa ke Kary Mullis oa Cetus, USA ka 1983. Mullis o ile a etsa kopo ea patent ea PCR ka 1985 'me a hatisa pampiri ea pele ea thuto ea PCR ka Saense ka eona ...Bala haholoanyane -
Ho fumanoa ha coronavirus e ncha, ho tloha ho nucleic acid, antibody ho ea ho antigen, mokhoa oa ho khetha tloaelo ea seoa?
Seoa se fetotse lefatše.Lefatšeng lohle, mebuso ea linaha tsohle e tobane le mathata a maholo a thibelo le taolo ea seoa.Nakong ea seoa sa COVID-19, China e methating e mene ea moralo oa thibelo le karabelo (thibelo, ho lemoha, taolo le Senotlolo sa ...Bala haholoanyane -
Mekhoa e mene ea ho thibela tšoaetso ea lihlahisoa tsa PCR
Ho aha SOP System Theha teko ea PCR SOP ho tiisa boitšoaro ba basebetsi ba liteko.Bahlahlobi ba latela mekhoa ea ts'ebetso ka tieo, 'me ba fokotsa tšilafalo ea PCR e ka bakoang ke mabaka a batho kapa ho thibela ho hlaha ha tšilafalo ts'ebetsong.Ho phaella moo...Bala haholoanyane -
Mokhoa oa ho ntlafatsa ts'ebetso ea phetisetso ea morao-rao ea LncRNA?
Lead Long e sa khoute RNA, lncRNA ke RNA e sa khoute e nang le bolelele bo fetang 200 nucleotide, hangata lipakeng tsa 200-100000 nt.lncRNA e laola liphatsa tsa lefutso maemong a epigenetic, transcript le post-transcriptional, hape e nka karolo ho khutsiseng X chromosome, ho hatisa genome le chroma...Bala haholoanyane -
E ka fetisoa ho tloha ho motho ho ea ho motho ka metsotsoana e 14.Ho etsahetse'ng ka "tšoaetso e fetelletseng" ea Delta e Zhong Nanshan Pass?
Mohloli o fetoletsoeng: Mohlophisi oa sehlopha sa WuXi AppTec Guangzhou, China, mapolesa a ikarabellang bakeng sa ho thusa lipatlisiso tsa lefu la seoa o ile a lokolla video ea leihlo: Lebenkeleng le le leng la reschorente, ba babeli ba ile ba kena ka kamoreng ea ho hlapela ka mor'a e mong ntle le ho kopana.Ke metsotsoana e 14 feela ea co-e...Bala haholoanyane -
SNP ho ngola le ho lemoha ha molek'hule
Ka mor'a hore setsebi sa Amerika Eric S. Lander se bue ka mokhoa o tloaelehileng oa "nucleotide polymorphism" (SNP) e le letšoao la moloko oa boraro oa limolek'hule ka 1996, SNP e 'nile ea sebelisoa haholo tlhahlobo ea mekhoa ea moruo, kaho ea liphatsa tsa lefutso tsa liphatsa tsa lefutso, le tlhahlobo ea liphatsa tsa lefutso tsa batho., D...Bala haholoanyane -
Saense e Tsebahalang |Mokhoa oa ho lemoha coronavirus e ncha ka qPCR
COVID-19 ke lefu le tšoaetsanoang le bakoang ke Severe Acute Respiratory Syndrome Coronavirus Type 2. Ha motho a tšoaelitsoe, matšoao a tloaelehileng a kenyelletsa feberu, ho khohlela, le ho hema ka thata.Mehlala e sebelisetsoang tlhahlobo e ka bokelloa ka li-swabs tsa nasopharyngeal kapa li-oropharyngeal swabs.Seo ke...Bala haholoanyane -
Na u ntse u sebelisa theknoloji e qapiloeng lilemo tse 30 tse fetileng ho ntša RNA?
Na u ntse u sebelisa Trizol ho ntša RNA?Oho, se ntumelle ho tšoenyeha ka bophelo ba hau!Trizol e haufi le ho qheleloa ka thoko, u se ke ua mpolella hore u ntse u le lefifing?Karolo e ka sehloohong ea reagent ea Trizol ke phenol, 'me li-reagents tsa organic tse kang chloroform le tsona li kenyelelitsoe tekong ...Bala haholoanyane
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