Mobu DNA Isolation Kit Extraction Purification Kits & Reagents for Soil DNA
Tlhaloso ea Kit:
Ka potlako hloekisa 'me u fumane DNA ea boleng bo holimo ho tsoa ho likokoana-hloko tse fapaneng tsa sampole ea mobu.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
Tlhaloso
Setsi sena se fana ka mokhoa o potlakileng le o bonolo oa ho ntša DNA ea genomic mehloling e fapaneng ea mobu.Ho na le palo e kholo ea li-inhibitors tse kang humic acid le li-ion tsa tšepe lisampoleng tsa mobu.Le haeba lintho tsena li le teng ka bongata ho DNA e hloekisitsoeng, li tla ba le tšusumetso liketsong tse tlase, joalo ka PCR le thibelo ea tšilo ea enzyme.Ka hona, senotlolo sa ho hloekisa DNA ea mobu ke mokhoa oa ho tlosa li-inhibitors mobung ka katleho.
Setsi sena se sebelisa DNA-Only Column e ka tlamang DNA ka ho khetheha, Foregene Protease e ncha le sistimi e ikhethang ea buffer, e ka tlosang li-inhibitors tse fapaneng mobung ntle le ho ntšoa ha motsoako oa manyolo kapa ethanol le pula ea isopropanol.Ho ntšoa ha DNA ho lisampole tsa mobu ho ka phethoa ka metsotso e 90.
Litlhaloso
50 Preps, 100 Preps, 250 Preps
Likarolo tsa Kit
| Buffer SG1 |
| Buffer SG2 |
| Buffer SG3 |
| Buffer SG4 |
| Sekhahla sa PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Foregene Protease |
| DNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
-Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho qoba laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
-Lebelo le potlakileng: Ts'ebetso e bonolo, mme ts'ebetso ea ho ntša DNA ea genomic ea mobu e ka phethoa ka hare ho metsotso ea 90.
-E bonolo: centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4°C centrifugation ea mocheso o tlase kapa pula ea ethanol ea DNA.
-Tshireletso: Ha ho hlokahale ho ntšoa ha reagent ea organic.
-Boleng bo phahameng: DNA e nkiloeng ea genomic e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse sa tšoaneng.
Sesebelisoa sa Kit
E loketse ho hloekisoa ha DNA ea genomic ho tloha lisampole tse latelang: lisampole tsa silt, mobu oa lipalesa tsa lipalesa, mobu o hatakelang, mobu oa moru, mobu oa lehoatata, mobu oa letamo, mobu o haufi le letša, mobu oa mohloa le mehlala e meng.
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
-Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C), haeba se hloka ho bolokoa nako e telele, se ka bolokoa ho 2-8 ° C.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.
-Forene Protease tharollo e na le mokhoa o ikhethang, o sebetsang ha o bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);mosebetsi oa eona le botsitso li tla ba molemo ha li bolokiloe ho 4 ° C, kahoo ho kgothaletswa ho e boloka ho 4 ° C, hopola hore u se ke ua e boloka ho -20 ° C.
-Dry Lysozyme phofo e bolokiloe ho -20 ° C;tharollo ea Lysozyme e lokiselitsoeng e arotsoe ka likaroloana tse nyenyane ebe e bolokoa ho -20 ° C.
