Peo ea Semela(Polysaccharide Polyphenol e ruileng, e kholo le e mahareng) Direct PCR Plus Kit II-UNG(ntle le lisebelisoa tsa Sampling)
Litlhaloso
Sehlahisoa sena se sebelisa mokhoa o ikhethang oa lysis reaction system, o ka lokollang DNA ea genomic ka potlako ho tloha lisampole tsa peo ea limela tse nang le li-polysaccharides tse phahameng le polyphenols (joaloka libanana, k'hothone, joalo-joalo) bakeng sa ho sebelisoa ka liketso tsa PCR, kahoo e loketse ka ho khetheha bakeng sa tlhahlobo e kholo ea liphatsa tsa lefutso.E le ho fihlela litlhoko tsa liteko tse fapaneng tsa ho lemoha, setsi sena se ka sebelisa mefuta e mengata ea lisampole (joalo ka peo e felletseng, maqeba a manyane, kapa lisampole tsa fatše tsa lipeo tse ngata) bakeng sa liteko.Har'a bona, bakeng sa lisampole tsa peo tse ntseng li hloka ho mela, o ka khetha ho khaola likotoana tsa li-micro-tissue (1-5mg) ntle le li-embryo tsa peo bakeng sa liteko;bakeng sa liteko tse hlokang lisampole le liteko ka palo e kholo ea lisampole, u ka khetha ho kopanya lisampole tse ngata le ho li kopanya.Ka mor'a ho sila ka likaroloana tse nang le bophara ba hoo e ka bang 0.5mm, teko e ka etsoa (bonyane 0.1% ea sampuli e reretsoeng e ka fumanoa).
Litlhaloso
50T,200T,500T,2000T
Likarolo tsa Kit
Plant Seed Direct PCR Plus Kit II Polysaccharide polyphenol semela peo (e kholo le e mahareng) e tobileng PCR kit | |||||
Sebopeho sa Kit | TP-0317T | TP-03171 | TP-03172 | TP-03173 | |
50 T | 200 T | 500 T | 2000 T | ||
Karolo ea I | Buffer SSP1 | 24ml | 96ml | 120ml × 2 | 480mlx2 |
Buffer SP2 | 3ml | 10ml | 25ml | 100ml | |
6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
Karolo ea II | Buffer SSP2 | 8ml | 32ml | 80ml | 80mlx4 |
2× Peō ea PCR e BonoloTMKopanya | 500μl | 1mlx2 | 1.7ml×3 | 1.7ml×12 | |
Bukana | 1 | 1 | 1 | 1 |
Likarolo le melemo
- Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.
- Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.
- Ha ho na liphekolo tse khethehileng tse kang ho sila le ho sila tse hlokahalang, 'me ts'ebetso e bonolo.
- Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
Mokhoa oa ho sebetsa
Maemo a polokelo
1.Lipalangoang
Ts'ebetso eohle ea lipalangoang tsa li-ice box tsa mocheso o tlase, ho etsa bonnete ba hore kit Karolo ea II e boemong ba <4 ℃.
2.Bobolokelo
Karolo ea I ea kit ena e lokela ho bolokoa mocheso oa kamore kapa 2-8 ° C.
Karolo ea II e lokela ho bolokoa ho -20°C.