Foregene DNA Identification System 38Y-C (Nyee)
Tlhaloso ea Kit:
Foregene Sistimi ea ho tsebahatsa DNA 38Y-C e sebelisa theknoloji ea mebala e tšeletseng ea ho ngola fluorescent ho holisa 38 Y chromosome STR sebaka, 3 Y-Indel lociletse peli tse ka hare ho laola boleng loci ka nako e le 'ngoebakeng sa ho holisa Mehlala ea linyeoe tse ntšitsoeng ho lithempleite tsa DNA tse hloekisitsoeng.
Tlhaloso
Foregene Sistimi ea ho tsebahatsa DNA 38Y-C e sebelisa theknoloji ea mebala e tšeletseng ea ho ngola fluorescent ho holisa 38 Y chromosome STR sebaka, 3 Y-Indel lociletse peli tse ka hare ho laola boleng loci ka nako e le 'ngoebakeng sa ho holisa Mehlala ea linyeoe tse ntšitsoeng ho lithempleite tsa DNA tse hloekisitsoeng.
Litaba tsa Kit
Tokisetso ea tsamaiso ea amplification
Thecompositio ea ho lokisetsan of Standardmotsuole tsamaiso ea amplification
| Master Mix | Cbahanyetsi | Sistimi ea 10μl (μl) |
| 2.5×PCR Master Mix C I | 4.0 | |
| 5 x 38Y-C Primer Mix | 2.0 | |
| Setšoantšo sa DNA | 1- 4 | |
| Metsi a deionized | Tlatsa ho fihlela bophahamo ba modumo ea10 μl |
Tlhahlobo ea Lintlha
Kerafo e Ekelitsoeng 1:'Mapa oa Allelic Ladder Typing
Kerafo e Ekelitsoeng 2:Tekanyetso ea ho thaepa ea DNA ea 9948'mapa
Reagent Storage
1. Ka kopo, li boloke ka tlase ho -20 ° C (haeba li sa sebelisoe ka nakoana) , ka mor'a ho amohela lisebelisoa tse leqhoa le leqhoa le omileng kapa liphutheloana tsa leqhoa la gel.
2. Ka kopo, sotre motsoako oa pele oa rection PCR master mix o ho -20 ° C, ka mor'a hore kit e ntšoe hore e sebelisoe 'me li-reagents tse setseng li bolokoa mocheso oa 4 ° C ho qoba ho ba leqhoa khafetsa le ho qhibiliha.Haeba tekanyo e le 'ngoe e le nyane, ho kgothaletswa ho boloka ho -20 ° C ka mor'a aliquoting.
3. Boloka likarolo ka mor'a karabelo ho 4 ° C, qoba ho qhoqhoa khafetsa le ho qhibiliha, 'me u se ke ua ama li-reagents pele u arabela ho qoba ho silafala.
