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PCR Purification Kit PCR Clean-up System Bakeng sa Ultra-pure DNA
Ka potlako hloekisa le ho fumana likhechana tsa DNA tsa boleng bo holimo ho tsoa ho sistimi ea PCR;haeba u batla ho arola le ho fumana likhechana tse khethehileng tsa DNA, ka kopo khetha lisebelisoa tsa ho khutlisa li-gel.
◮ Mefuta e mengata ea ho hlaphoheloa ha DNA: Likaroloana tsa DNA tse khutšoane joalo ka 60bp le tse kholo joalo ka 10kb li ka fumanoa.
◮ Ts'ebetso e phahameng ea ho hlaphoheloa: Ts'ebetso ea ho hlaphoheloa hangata e feta 80%, 'me e phahameng ka ho fetisisa e ka fihla ho feta 95%.
◮ Lebelo le potlakileng:Ho bonolo ho sebetsa, ho hlaphoheloa ha sekhechana sa DNA ho ka phethoa nakong ea metsotso e 15 mme primer dimer e ka tlosoa ka kotloloho ntle le ho hlaphoheloa ha gel.
◮ Safety: Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮ Boleng bo phahameng: Likaroloana tse fumanoeng tsa DNA li hloekile haholo, tse ka kopanang le liteko tse fapaneng tse latelang.
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Baktheria DNA Isolation Kit Bacterial Genomic DNA Extraction Purification Kits
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa ho libaktheria karolong ea kholo ea logarithmic.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits ho tloha Mouse Tail
Thepa e potlakileng ka ho fetisisa lefatšeng ea ho ntša DNA ea mohatla oa mouse, e ka ntšang DNA ea boleng bo holimo mohatleng oa toeba nakong ea hora e le 'ngoe.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Gel Extraction Kit DNA Gel Extraction Kit
Ka potlako le ka bokhabane fumana likhechana tsa DNA tsa 20bp-10kb ho jeleng ea agarose.
◮Mefuta e mengata ea ho hlaphoheloa ha DNA:Likaroloana tsa DNA tse khutšoane joalo ka 30bp le tse kholo joalo ka 10kb li ka fumanoa.
◮Bokhoni bo phahameng ba ho hlaphoheloa:Ts'ebetso e phahameng ka ho fetisisa ea ho hlaphoheloa e ka fihla ho feta 80%.
◮Khatiso e nyane ea sistimi:bonyane 30μl elution tharollo ka sebelisoa bakeng sa elution, e leng ka ka katleho eketsa mahloriso ea a hlaphoheloa likhechana tsa DNA.
◮Lebelo le potlakileng:Ho bonolo ho sebetsa, ho hlaphoheloa ha sekhechana sa DNA ho ka phethoa nakong ea metsotso e 15.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:Likaroloana tse fumanoeng tsa DNA li hloekile haholo, tse ka kopanang le liteko tse fapaneng tse latelang.
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EndoFree Maxi Plasmid Kit (Spin Column)
Ts'ebetso eohle ea ho ntša e nka hora e le 'ngoe feela, ho netefatsa ts'ebetso e bonolo le e potlakileng.
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