Foregene DNA Identification System 20A (Mahala DNA Extraction)
Tlhaloso ea Kit:
Foregene DNA identification system 20A e sebelisa theknoloji ea mebala e mehlano ea fluorescent ho holisa ka nako e le 'ngoe 19 STR loci le 1 ea thobalano ka har'a tube e le' ngoe.Ha e ntse e akaretsa 13 CODIS core loci, 20 core loci kopana le litlhoko tsa Lekala la Tšireletso ea Sechaba bakeng sa autosomal STR kit.Ke monna ea ka letsohong le letona oa kaho ea database ea forensic DNA le boitsebahatso ba leloko la boahloli.
Tlhaloso
Foregene DNA identification system 20A e sebelisa theknoloji ea mebala e mehlano ea fluorescent ho holisa ka nako e le 'ngoe 19 STR loci le 1 ea thobalano ka har'a tube e le' ngoe.Ha e ntse e akaretsa 13 CODIS core loci, 20 core loci kopana le litlhoko tsa Lekala la Tšireletso ea Sechaba bakeng sa autosomal STR kit.Ke monna ea ka letsohong le letona oa kaho ea database ea forensic DNA le boitsebahatso ba leloko la boahloli.
Litaba tsa Kit
| Sehlopha sa Kit | Lebitso la Motsoako | Bolumo (μl / tube) | Bongata (tube) |
| PCR Pre-reaction reagent | 2.5×PCR karabelo buffer Ⅲ | 1000 | 2 |
| 5 × 20A motsoako oa pele | 500 | 2 | |
| Laola DNA 9947A (1ng/μl) | 25 | 1 | |
| Metsi a deionized | 1700 | 2 | |
| Taq Polymerase | Taq polymerase Ⅲ | 40 | 2 |
| PCR Rear-reaction reagent | Allelic ladder20A | 40 | 1 |
| Boima ba molek'hule ka hare ho standardORG 500 | 150 | 2 | |
| Hlokomela | |||
Tlhahlobo ea Lintlha
1. Bula software ea Genemapper ID.Ha u sebelisa Kit ea pele, u hloka ho kenya lipanete, 'me u behiloe, tse 30, tse 380, tse 380, 420, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, 490, ya 460, ya 400)
2. Kenya lintlha tsa electrophoresis, khetha litekanyetso tse lumellanang tsa tlhahlobo tse kang phanele, mokhoa oa ho hlahloba, le tekanyo ea boholo, 'me u fetole mofuta oa sampuli ea Lere ho "Allelic Ladder" kholomong ea "Mofuta oa Mohlala";qala ho sekaseka data.
Reagent Storage
1. Ka mor'a ho amohela kit e leqhoa ka leqhoa le omileng kapa lipakete tsa leqhoa tsa polasetiki, haeba li sa sebelisoe ka nakoana, ka kopo li boloke ka tlase ho -20 ° C nako e telele.
2. Ka mor'a hore setlolo se ntšoe 'me se sebelisoe, sekoti se lokela ho bolokoa ka 4 ° C pele ho karabelo ho qoba ho qhoqhoa khafetsa le ho qhibiliha;Taq enzyme e lokela ho bolokoa ho -20 ° C.
3. Ka mor'a karabelo, kit (ho kenyeletsoa tekanyo ea ka hare ea boima ba molek'hule le lere) e lokela ho bolokoa "kamoreng ea ho lemoha electrophoresis" ka 4 ° C, qoba ho qhoqhoa khafetsa le ho qhibiliha, 'me u qobe ho kopana le lisebelisoa tsa pele ho ho etsa lintho ho qoba ho silafala.
