Foregene DNA Identification System 27Y
Tlhaloso ea Kit:
Foregene DNA Identification System 27Y e sebelisa thekenoloji ea ho ngola ka mebala e tšeletseng ea fluorescent ho holisa 27 Y-STR loci ka nako e le 'ngoe.Ho kenyeletsoa: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS39, DY398, DY398 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Kit e na le bokhoni bo botle ba ho thibela ho thibela, 'me e ka ntlafatsa ka ho toba pampiri ea ho hloekisa, karete ea FTA, swab ea k'hothone, karete ea saliva, oral swab,' me e ka boela ea sebelisoa ho ntša le ho hloekisa litempele tsa DNA ho tloha thepa ea linyeoe.
Tlhaloso
Foregene DNA Identification System 27Y e sebelisa thekenoloji ea ho ngola ka mebala e tšeletseng ea fluorescent ho holisa 27 Y-STR loci ka nako e le 'ngoe.Ho kenyeletsoa: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS39, DY398, DY398 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Kit e na le bokhoni bo botle ba ho thibela ho thibela, 'me e ka ntlafatsa ka ho toba pampiri ea ho hloekisa, karete ea FTA, swab ea k'hothone, karete ea saliva, oral swab,' me e ka boela ea sebelisoa ho ntša le ho hloekisa litempele tsa DNA ho tloha thepa ea linyeoe.
Litaba tsa Kit
| Setsi sa tlhahlobo ea reagent | Lebitso la karolo | Bolumo (μl/tube) | Bongata (tube) |
| PCR pre-rection kit | 2 × PCR Reaction Master Mix | 1250 | 2 |
| 5 x 27Y Primer Mix | 500 | 2 | |
| DNA Typing Standard 9948 | 20 | 2 | |
| Metsi a deionized | 1700 | 2 | |
| Post-PCR Reaction Kit | 27Y allele motsoako Lere | 20 | 2 |
| Orange molek'hule boima ba ka hare tekanyetso ORG500 | 150 | 2 |
Tlhahlobo ea Lintlha
1. Bula software ea Gene Mapper ID.Ha u sebelisa tloko ena ka lekhetlo la pele, u hloka ho kenya lipane, 'me u behiloe, tse 30, tse 380, 390, 490, 490, 490, 490, 560, 560, 560, 560, 560, 560, 560, 560, 560, 560, 560, 560, 560, 560, 560, 560, 560, 390, 490, 390, ya 380
2. Kenya lintlha tsa electrophoresis, khetha litekanyetso tse lumellanang tsa tlhahlobo tse kang phanele, mokhoa oa ho hlahloba, le tekanyo ea boholo, 'me u fetole mofuta oa sampuli ea Lere ho "Allelic Ladder" kholomong ea "Mofuta oa Mohlala";qala ho sekaseka data.
Reagent Storage
1. Ka kopo li boloke ka tlase ho -20 ° C (haeba li sa sebelisoe ka nakoana), ka mor'a ho amohela li-kits tse leqhoa le leqhoa le omileng kapa li-gel ice packs.
2. Ka kopo boloka kit ka 4 ° C pele ho karabelo ho qoba ho qhoqhoa khafetsa le ho qhibiliha, ka mor'a hore kit e ntšoe hore e sebelisoe,;enzyme e lokela ho bolokoa ho -20 ° C.
2. Ka mor'a karabelo, kit (ho kenyeletsoa tekanyo ea ka hare ea boima ba molek'hule le lere) e lokela ho bolokoa "kamoreng ea ho lemoha electrophoresis" ka 4 ° C, qoba ho qhoqhoa khafetsa le ho qhibiliha, 'me u qobe ho kopana le lisebelisoa tsa pele ho ho etsa lintho ho qoba ho silafala.
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