Foregene DNA Identification System 25A-C (Nyee)
Tlhaloso ea Kit:
Foregene DNA Identification System 25A-C e sebelisa thekenoloji ea mebala e tšeletseng ea ho ngola fluorescence ho holisa 23 autosomal STR loci, Amel loci e le 'ngoe, e le' ngoe ea Yindel loci le libaka tse peli tsa taolo ea boleng ba ka hare ka nako e le 'ngoe bakeng sa ho holisa li-template tsa DNA tse ntšitsoeng le ho hloekisoa ho tloha lisampoleng tsa linyeoe.
Tlhaloso
Foregene DNA Identification System 25A-C e sebelisa thekenoloji ea mebala e tšeletseng ea ho ngola fluorescence ho holisa 23 autosomal STR loci, Amel loci e le 'ngoe, e le' ngoe ea Yindel loci le libaka tse peli tsa taolo ea boleng ba ka hare ka nako e le 'ngoe bakeng sa ho holisa li-template tsa DNA tse ntšitsoeng le ho hloekisoa ho tloha lisampoleng tsa linyeoe.
Litaba tsa Kit
Tokisetso ea tsamaiso ea amplification
The Preparation Composition of Standard extraction and amplification system
| Likaroloana | Sistimi ea 10μl(μl) | |
| Master Mix | 2.5×PCR Premix C Ⅰ | 4.0 |
| 10×25A-CPrimer Mix | 1.0 | |
| Setšoantšo sa DNA | 1—5 | |
| Metsi a deionized | Etsa bophahamo ba molumo oa 10μl |
Tlhahlobo ea Lintlha
Kerafo e Ekelitsoeng 1:Lere genotyping ea allele
Kerafo e Ekelitsoeng 2:Tekanyetso ea ho thaepa ea DNA ea 9948'mapa
Reagent Storage
1. Kamora ho amohela kit, ka kopo e boloke ka tlase ho -20 ℃ haeba u sa e sebelise hajoale.
2. Ka mor'a hore kit e ntšoe 'me e sebelisoe, karolo ea pele ea karabo ea PCR premixed solution e bolokoa ho -20 ℃, 'me li-reagents tse setseng pele li bolokiloe li bolokiloe ho 4 ℃ ho qoba ho qhibiliha ka makhetlo a mangata.Haeba tekanyo e le 'ngoe e le nyenyane, ho kgothaletswa ho boloka ho -20 ° C ka mor'a ho paka.
3. Kamora karabelo, likarolo li bolokoa ho 4 ℃ ho qoba ho qhibiliha khafetsa.Se ke oa ama li-reagents tsa pre-reaction ho qoba tšilafalo.
