Foregene DNA Identification System 30A (Non-Extraction)
Tlhaloso ea Kit:
FOREGENE DNA identification system 30A e sebelisa theknoloji ea mebala e tšeletseng ea ho ngola fluorescent hoholisa 29autosomalSSebaka sa TR, ngoeLocus ea Amel le sebaka se le seng sa Yindel ka nako e le 'ngoe,' me e ka holisa pampiri ea sefa ka kotloloho, FTAlikarete, li-swabs tsa k'hothone, likarete tsa mathe,le mswab ea kantle.
Tlhaloso
FOREGENE DNA identification system 30A e sebelisa theknoloji ea mebala e tšeletseng ea ho ngola fluorescent hoholisa 29autosomalSSebaka sa TR, ngoeLocus ea Amel le sebaka se le seng sa Yindel ka nako e le 'ngoe,' me e ka holisa pampiri ea sefa ka kotloloho, FTAlikarete, li-swabs tsa k'hothone, likarete tsa mathe,le mswab ea kantle.
Litaba tsa Kit
Tokisetso ea Sistimi ea Amplification
Letlapa la 1: Sebopeho sa boitokisetso sa Maiketsetso a amplification a Maemo a Maemo (Mahala a ho ntšitsoeng).
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| Cbahanyetsi | 25 Sistimi ea μl (μl) | 10 Sistimi ea μl (μl) |
| Master Mix | 4 × Master Mix VII | 6.25 | 2.5 |
| 5 x 30APrimer Mix | 5.0 | 2 | |
| Metsi a deionized | 13.75 | 5.5 | |
| Bthupa letheba | Bophara ba 1.2mm | Bophara ba 1.2mm | |
| Tmolumo oa otal reaction | 25 μl | 10 μl | |
Tlhahlobo ea Lintlha
Kerafo e Ekelitsoeng 1:'Mapa oa Allelic Ladder Typing
Kerafo e Ekelitsoeng 2:Tekanyetso ea ho thaepa ea DNA ea 9948'mapa
Reagent Storage
1. Ka kopo boloka ka tlase ho -20 ° C, ka mor'a ho amohela lisebelisoa tse leqhoa le leqhoa le omileng kapa liphutheloana tsa leqhoa la gel.
2. Ka kopo boloka karolo ea pre-reaction 4 × premix VII ho -20 ° C, ka mor'a hore kit e ntšoe bakeng sa ho sebelisoa, 'me u boloke li-reagents tse ling tse setseng pele ho 4 ° C ho qoba ho qhoqhoa khafetsa le ho qhibiliha.Haeba tekanyo e le 'ngoe e le nyane, ho kgothaletswa ho boloka ho -20 ° C ka mor'a ho qojoa.
3. Boloka likarolo ka mor'a karabelo ho 4 ° C, qoba ho qhoqhoa khafetsa le ho qhibiliha, 'me u se ke ua ama li-reagents pele u arabela ho qoba ho silafala.
