Foregene DNA Identification System 30 AC (Nyee)
Tlhaloso ea Kit:
Foregene Sistimi ea ho tsebahatsa DNA30A-C e sebelisa theknoloji ea ho ngola ka mebala e tšeletseng ea fluorescent hoholisa 29autosomalSTR sebaka, 1 Amel locus, 1 Yindellocus, le1 Libaka tse ikhethileng tsa batho tsa Thobalano, 2libaka tsa taolo ea boleng ba ka hare, tse sebelisetsoang ho holisaDNAtemplate e ntšitsoeng le ho hloekisoa ho tlohaClisebelisoa tsa ase.
Tlhaloso
Foregene Sistimi ea ho tsebahatsa DNA30A-C e sebelisa theknoloji ea ho ngola ka mebala e tšeletseng ea fluorescent hoholisa 29autosomalSTR sebaka, 1 Amel locus, 1 Yindellocus, le1 Libaka tse ikhethileng tsa batho tsa Thobalano, 2libaka tsa taolo ea boleng ba ka hare, tse sebelisetsoang ho holisaDNAtemplate e ntšitsoeng le ho hloekisoa ho tlohaClisebelisoa tsa ase.
Litaba tsa Kit
Tokisetso ea tsamaiso ea amplification
Letlapa la 2: The Preparation Composition of Standard extraction and amplification system
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| Cbahanyetsi | Sistimi ea 25ul (µl) | Sistimi ea 10μl (μl) |
| Master Mix | 2.5× PremixCⅡ | 10.0 | 4.0 |
| 5 x 30 AC Primer Mix | 5.0 | 2.0 | |
| Setšoantšo sa DNA | 2.5-10 | 1-4 | |
| Metsi a deionized | Etsa bophahamo ba molumo oa 25μl | Etsa ho fihlela bophahamo ba modumo ea10 μl |
Tlhahlobo ea Lintlha
Kerafo e Ekelitsoeng 1:'Mapa oa Allelic Ladder Typing
Kerafo e Ekelitsoeng 2:Tekanyetso ea ho thaepa ea DNA ea 9948'mapa
Reagent Storage
1. Ka kopo boloka ka tlase ho -20 ° C, ka mor'a ho amohela lisebelisoa tse leqhoa le leqhoa le omileng kapa liphutheloana tsa leqhoa la gel.
2. Ka kopo boloka karolo ea pre-reaction 2.5 × premix CII ho -20 ° C ka mor'a hore kit e ntšoe hore e sebelisoe, 'me li-reagents tse setseng pele li bolokiloe li bolokiloe ho 4 ° C ho qoba ho qhoqhoa le ho qhibiliha khafetsa.Haeba tekanyo e le 'ngoe e le nyane, ho kgothaletswa ho boloka ho -20 ° C ka mor'a aliquoting.
3. Boloka likarolo ka mor'a karabelo ho 4 ° C, qoba ho qhoqhoa khafetsa le ho qhibiliha, 'me u se ke ua ama li-reagents pele u arabela ho qoba ho silafala.
