Semela sa DNA Isolation Kit Genomic Plant DNA Purification Kits Reagents Protocol
Litlhaloso
50 Preps, 100 Preps, 250 Preps
Setsi sena se sebelisa kholomo ea DNA feela e ka kopanyang DNA, Foregene protease le sistimi e ikhethang ea buffer, e nolofatsang haholo tlhoekiso ea DNA ea genomic ea semela.DNA ea boleng bo holimo e ka fumanoa nakong ea metsotso e 30, e qobang ho senyeha ha DNA ea genomic.
The DNA-feela silica gel lera sebelisoa ka ho spin kholomo ke Foregene e ncha e ikhethang lintho tse bonahalang, e ka ka tsela e atlehileng le ka ho toba tlama ho DNA, 'me ka ho fetisisa ho tloswa ha RNA, litšila liprotheine, ion, polysaccharides, polyphenols le metsoako e meng ea manyolo.
Likarolo tsa sehlahisoa
| Buffer PL1, Buffer PL2 |
| Buffer PW, Buffer WB, Buffer EB |
| Foregene Protease |
| DNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
■ Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le RNase e eketsehileng nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
■ Lebelo le potlakileng: Foregene Protease e na le tšebetso e phahameng ho feta ea protease e tšoanang ’me e sila lisampole tsa linama kapele.
■ E bonolo: ts'ebetso ea ho ntša DNA ea genomic e ka phethoa ka metsotso e 30.
■ Bonolo: The centrifugation e etsoa mocheso oa kamore, ha ho 4℃ mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA e hlokahalang.
■ Tšireletseho: ha ho na reagent ea tlhaho e hlokahalang.
■ Boleng bo phahameng: DNA e hloekisitsoeng ea genomic e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlaase haholo, li ka finyella litlhoko tsa liteko tse fapa-fapaneng.
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha DNA ea genomic ho tloha liseleng tse ncha kapa tse leqhoa.
Phallo ea mosebetsi
Setšoantšo
Storage le Shelf bophelo
Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) le 2-8 ℃ nako e telele.
Foregene Protease Plus tharollo e na le foromo e ikhethang, e sebetsang ha e bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);mosebetsi oa eona le botsitso li tla ba molemo ha li bolokiloe ho4 ℃, kahoo ho kgothaletswa ho e boloka ho 4 ℃, hopola hore o seke oa e boloka ho -20 ℃.
Tataiso ea Tlhahlobo ea Mathata
The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.
Kotulo e tlase kapa ha e na DNA
Hangata ho na le lintlha tse ngata tse amang tlhahiso ea DNA ea genomic, ho kenyeletsa mohloli oa sampuli, lilemo tsa sampuli, maemo a polokelo ea sampuli, le ts'ebetso.
Genomic DNA e ne e ke ke ea fumanoa nakong ea ho ntšoa
1. Lisampole tsa linama li bolokoa ka tsela e fosahetseng kapa li bolokoa nako e telele haholo, e leng se bakang ho senyeha ha DNA ea genomic.
Keletso: Boloka disampole tsa dithishu ka nitrogen e metsi kapa -20°C;leka ho sebelisa lisampole tse sa tsoa bokelloa bakeng sa ho ntšoa ha DNA ea genomic.
2. Chelete e nyane haholo ea sampole e ka etsa hore DNA e tsamaisanang le eona e se ke ea ntšoa.
Tlhahiso: Bakeng sa lisampole tsa linama tse bolokiloeng nako e telele kapa tse nang le ho senyeha ho matla ha DNA ea genomic, palo ea lisampole tsa linama li ka eketsoa ka nepo molemong oa ho ntša DNA e ngata.Palo ea sampole e ka khethoa ho latela litlhoko tsa DNA, empa sampole e ncha ha ea lokela ho feta 100mg, mme sampole e omeletseng ha ea lokela ho feta 30mg.
3. Sampole ha e siloe ka naetrojene e metsi kapa e behoa nako e telele haholo ka mor'a metsi a naetrojene.
Tlhahiso: Nakong ea ho ntšoa ha DNA, sampole e hloka ho siloa ka botlalo ka metsi a naetrojene ho pshatla lerako la sele;ka mor'a ho sila, ka kopo fetisetsa phofo ea sampuli ho PL1 e futhumetseng pele ho 65°C kapele kamoo ho ka khonehang (hang ha phofo ea fatše e qhibiliha, DNA ea genomic e tla qala ho senyeha ka potlako) .
4. Poloko e fosahetseng ea Foregene Protease e fella ka ts'ebetso e fokotsehileng kapa e sa sebetseng.
Keletso: Netefatsa maemo a polokelo ya Foregene Protease kapa o e nkele sebaka ka Foregene Protease e ntjha bakeng sa enzymatic hydrolysis.
5. Sesebelisoa se bolokoa ka mokhoa o sa lokelang kapa se bolokiloe nako e telele haholo, se etsa hore likarolo tse ling tsa kit li hlōlehe.
Keletso: Reka sesebelisoa se secha sa genomic DNA extraction bakeng sa ts'ebetso e amanang.
6. Tšebeliso e fosahetseng ea kit.
Tlhahiso: Reka Plant DNA Isolation Kit e inehetseng ho disampole bakeng sa ho ntšoa le ho hloekisa DNA ea semela sa genomic.
7. Buffer WB ntle le ho eketsa ae se nang metsi ethanol.
Keletso: Etsa bonnete ba hore o eketsa molumo o nepahetseng oa ethanol ho Buffer WB.
8. The elent e ne e sa rothele holim'a lera la silica ka nepo.
Tlhahiso: Eketsa tlhahiso-pele e futhumetseng ho 65℃dropwise ho ea bohareng ba lera la silika la gel, 'me u le tlohele mocheso oa kamore metsotso e 5 ho eketsa katleho ea elution.
Ho ntšoa ho fumana DNA ea genomic ea lihlahisoa tse tlase
1. Mohlala o bolokoa ka tsela e fosahetseng kapa o bolokoa nako e telele haholo, e leng se bakang ho senyeha ha genomic DNA.
Keletso: Boloka disampole tsa dithishu ho -20℃;leka ho sebelisa lisampole tsa linama tse sa tsoa bokelloa bakeng sa ho ntšoa ha DNA ea genomic.
2. Haeba palo ea lisampole tsa lisele e le nyenyane haholo, DNA e nkiloeng ea genomic e tla fokotseha.
Tlhahiso: Mehlala e meng ea limela e na le metsi a mangata, joalo ka limela tsa metsing tse kang algae, joalo-joalo, tekanyo e ka eketsoa ka nepo kapa metsi a ka felloa ke metsi hanyane pele ho ts'ebetso.
3. Mehlala ha ea ka ea siloa hantle ka naetrojene e metsi kapa e ile ea siuoa mocheso oa kamore nako e telele haholo ka mor'a ho sila.
Tlhahiso: Mokelikeli oa naetrojene o silang o tlameha ho lekana, 'me lerako la lisele tsa mohlala le lokela ho robeha ka hohle kamoo ho ka khonehang;hang ka mor'a ho sila, phofo ea sampole e lokela ho fetisetsoa ho 65℃preheated Buffer PL1 bakeng sa mohato o latelang.
4. Ho se sebelise khiti e nepahetseng.
Keletso: Sebelisa Semela se Isolation sa Plant DNA ho hula le ho hloekisa DNA ea semela.
5. Poloko e fosahetseng ea Foregene Protease e fella ka ts'ebetso e fokotsehileng kapa e sa sebetseng.
Keletso: Netefatsa maemo a polokelo ya Foregene Protease kapa o e nkele sebaka ka Foregene Protease e ntjha bakeng sa enzymatic hydrolysis.
6. Bothata ba Eluent
Keletso: Ka kopo sebelisa Buffer EB bakeng sa elution;haeba o sebelisa ddH2O kapa lintlha tse ling, etsa bonnete ba hore pH ea leseli e pakeng tsa 7.0-8.5.
7. Theuent ha e rothiloe ka nepo
Tlhahiso: Ka kopo, eketsa lerotholi la elution bohareng ba lera la silika 'me u le tlohele mocheso oa kamore metsotso e 5 ho eketsa katleho ea elution.
8. Molumo oa molumo o monyenyane haholo
Tlhahiso: Ka kopo sebelisa leseli bakeng sa tlhabollo ea genomic DNA ho latela litaelo, bonyane eseng ka tlase ho 100.μl.
DNA e nkiloeng ea genomic e nang le bohloeki bo tlaase
Bohloeki bo fokolang ba DNA ea genomic bo tla lebisa ho hlōleha kapa phello e mpe ea liteko tse tlaase, tse kang: enzyme e ke ke ea khaoloa, 'me sekhechana sa liphatsa tsa lefutso se ke keng sa fumanoa ke PCR.
1. Tšilafalo ea liprotheine tse fapa-fapaneng, tšilafalo ea RNA.
Analysis: Buffer PW ha ea sebelisoa ho hlatsoa kholomo;Buffer PW ha ea sebelisoa ho hlatsoa kholomo ka lebelo le nepahetseng la centrifugation.
Tlhahiso: leka ho etsa bonnete ba hore ha ho na pula ho matla a phahametseng tlhaho ha matla a phahametseng a feta kholomong;etsa bonnete ba hore u hlatsoa kholomo ea tlhoekiso ka Buffer PW ho latela litaelo, 'me mohato ona o ke ke oa tloheloa.
2. Tšilafalo ea ion tšilafalo.
Tlhahlobo: Kholumo ea ho hlatsoa ea Buffer WB e siiloe kapa e hlatsuoa hanngoe feela, e leng se ileng sa baka tšilafalo e setseng ea ionic.
Keletso: Etsa bonnete ba hore o hlatsoa habeli ka Buffer WB ho latela litaelo tsa ho tlosa li-ion tse setseng ka hohle kamoo ho ka khonehang.
3. Tšilafalo ea RNase.
Analysis: Exogenous RNase e eketsoa ho Buffer;ts'ebetso e fosahetseng ea ho hlatsoa ho Buffer PW e tla fella ka RNase e setseng le ho ama tšebetso ea liteko ea RNA e tlase, joalo ka mongolo oa in vitro.
Tlhahiso: Foregene series nucleic acid extraction kits e ka tlosa RNA ntle le RNase e eketsehileng, le li-reagents tsohle tse ho Plant DNA Isolation Kit ha li hloke RNase;etsa bonnete ba hore o hlatsoa kholomo ea tlhoekiso ka Buffer PW ho latela litaelo, mme mohato ona o ke ke oa tloheloa.
4. Mesaletsa ea Ethanol.
Tlhahlobo: Ka mor'a ho hlatsoa kholomo ea tlhoekiso ka Buffer WB, ha ho letho le entsoeng ka tube centrifugation.
Keletso: Latela litaelo bakeng sa centrifugation e nepahetseng e se nang letho.
Buka ea litaelo:
Semela DNA Isolation Kit Tataiso ea Tataiso
