Genomic DNA Micro Kit Micro Genomic DNA Mini Kit
Tlhaloso ea Kit:
Ka potlako hloekisa lisampole tse nyane ho fumana DNA ea boleng bo holimo.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
Tlhaloso
Setsi sena se sebelisa DNA-Feela Column e ka kopanyang DNA, Foregene Protease e ncha le sistimi e ikhethileng ea buffer, e ka ntšang lisampole tse fapaneng tsa tlhaho, joalo ka palo ea mali, lisele le liphoofolo nakong ea metsotso e 20-80.Lithapo, matheba a mali a omisitsoeng, li-swabs tsa k'hothone tsa kliniki, li-follicles tsa moriri, joalo-joalo, li hloekisoa ho fumana DNA ea genomic ea boleng bo phahameng, ea boleng bo phahameng.
Litlhaloso
50 Preps
Likarolo tsa Kit
| Sekhahla sa ST1 |
| Buffer ST2* |
| Buffer PW* |
| Buffer WB |
| Buffer EB |
| Foregene Protease |
| DNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
-Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
-Lebelo le potlakileng: Foregene Protease e na le ts'ebetso e phahameng ho feta protease e tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
-E bonolo: The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlaase oa centrifugation kapa ethanol precipitation ea DNA.
-Tshireletso: Ha ho hlokahale ho ntšoa ha reagent ea organic.
-Boleng bo phahameng: DNA e nkiloeng ea genomic e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse sa tšoaneng.
-Micro-elution system: E ka eketsa bongata ba DNA ea genomic, e leng bonolo bakeng sa ho lemoha kapa ho etsa liteko.
Sesebelisoa sa Kit
E loketse ho hloekisoa ha lisampole tse latelang tsa DNA ea genomic: lithane tse ncha kapa tse hatselitsoeng tsa liphoofolo, palo e ncha kapa ea anticoagulated cryopreserved ea mali a felletseng, matheba a omisitsoeng a mali, lisele tse hlahisitsoeng, lisele tsa microsection, follicles ea moriri, melomo, joalo-joalo.
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
-Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho 2-8°C.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.
-Forene Protease tharollo e na le mokhoa o ikhethang, o sebetsang ha o bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);ts'ebetso ea eona le botsitso li tla ba betere ha li bolokiloe ho 4°C, kahoo ho kgothaletswa ho boloka ho4°C, hopola hore u se ke ua e boloka ho -20°C.
