Semela Kakaretso ea RNA Isolation Plus Kit
Tlhaloso ea Kit:
Tlosa DNA ka katleho u sebelisa DNA-Cleaning Column
Tlosa DNA ntle le ho eketsa DNase
E bonolo - ts'ebetso eohle e phethoa ka mocheso oa kamore
Ka potlako-ts'ebetso e ka phethoa ka metsotso e 30
E bolokehile - ha ho na reagent ea tlhaho e sebelisoang
Litlhaloso
50 Preps, 200 Preps
Thepa e sebelisa "spin column le formula" e ntlafalitsoeng ke Foregene, e ka ntšang RNA ea boleng bo holimo le ea boleng bo holimo ho lithane tse fapaneng tsa semela tse nang le li-polysaccharides tse phahameng kapa polyphenols.E fana ka kholomo ea DNA-Cleaning e ka tlosang DNA ea genomic habonolo ho tsoa ho supernatant le lithane tsa lysate.Kholomo ea RNA feela e ka tlama RNA ka nepo.Kit e ka sebetsana le palo e kholo ea lisampole ka nako e le 'ngoe.
Tsamaiso eohle ha e na RNase, kahoo RNA e hloekisitsoeng e ke ke ea senyeha.Buffer PRW1 le Buffer PRW2 e ka etsa bonnete ba hore RNA e fumanoeng ha e silafatsoe ke protheine, DNA, ion, le metsoako ea tlhaho.
Likarolo tsa Kit
| Buffer PSL1, Buffer PS, Buffer PSL2 |
| Buffer PRW1, Buffer PRW2 |
| RNase-Free ddH2O, DNA-Cleaning Column |
| Lekholomo la RNA Feela |
Likarolo le melemo
■ Ts'ebetso ka mocheso oa kamore (15-25 ℃) ho pholletsa le ts'ebetso eohle, ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Complete Kit RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ E loketse ka ho khetheha bakeng sa tlhoekiso ea RNA ho tloha lisampole tsa limela tsa polysaccharides le polyphenols.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore kit e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase.
■ Poelo e phahameng ea RNA: Kholomo ea RNA-feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa nakong ea metsotso e 30.
■ Tšireletseho: ha ho na reagent ea tlhaho e hlokahalang.
■ Boleng bo phahameng: Likaroloana tsa RNA tse hloekisitsoeng li hloekile haholo, ha li na protheine le litšila tse ling, 'me li ka kopana le mekhoa e fapaneng ea liteko.
Lihlahisoa tsa lihlahisoa
■ Lisebelisoa tse tlase: motsoako oa pele oa cDNA, RT-PCR, cloning ea molek'hule, Northern Blot, joalo-joalo.
■ Mohlala: Lithishu tsa semela tse ncha kapa tse hatselitsoeng tsa polysaccharides le polyphenols
■ Tekanyetso: 50mg dithishu tsa semela
■ Boholo bo tlamang ba RNA ea kholomo ea tlhoekiso: 80 μg
■ Molumo oa elution: 50-200 μl
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha kakaretso ea RNA ho tloha lisampoleng tse foreshe kapa tse hatselitsoeng (haholo-holo mahlaku a mahlaku a semela) a nang le polysaccharide e phahameng le polyphenol.
Phallo ea mosebetsi
Setšoantšo
Plant Total RNA Isolation Kit Plus e sebelitse 50mg ea makhasi a macha a polysaccharides le polyphenols, 'me 5% e hloekisitsoeng ea RNA e ile ea lekoa ke electrophoresis.
1: Banana
2: Ginkgo
3: K'hothone
4: Kharenate
Storage le Shelf bophelo
Setsi sena se ka bolokoa likhoeli tse 24 tlas'a maemo a omileng mocheso oa kamore (15-25 ℃);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ka 2–8℃.
Buffer PSL1 e ka behoa ho 4℃ bakeng sa khoeli e le 1 ka mor'a ho eketsa β-mercaptoethanol (ho kgothaletswa ho e kenyelletsa ka nako e le nngwe ya teko).
