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ForeDirect RT-qPCR Kit
◮Bakeng sa ntlafatso ea nako ea 'nete ea RNA ka kotloloho ho tsoa ho likoleke tsa swab ntle le lits'ebetso tsa tlhoekiso ea RNA.Sesebelisoa sena se phethela lipotoloho tsa qRT-PCR nakong ea hora e le 'ngoe.Motsoako oa enzyme ke motsoako o ntlafalitsoeng oa Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Reaction Buffer e na le likarolo tsohle tse hlokahalang, ho kenyeletsoa likarolo tsa buffer tse ntlafalitsoeng, Mg2+, dUTP, le dNTPs.
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FFPE DNA Isolation Kit DNA Extraction Purification Kit ho tloha FFPE Samples
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa ho lithane tse kentsoeng ke parafun tse kang likarolo tsa parafun le liboloko tsa parafun.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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PCR Purification Kit PCR Clean-up System Bakeng sa Ultra-pure DNA
Ka potlako hloekisa le ho fumana likhechana tsa DNA tsa boleng bo holimo ho tsoa ho sistimi ea PCR;haeba u batla ho arola le ho fumana likhechana tse khethehileng tsa DNA, ka kopo khetha lisebelisoa tsa ho khutlisa li-gel.
◮ Mefuta e mengata ea ho hlaphoheloa ha DNA: Likaroloana tsa DNA tse khutšoane joalo ka 60bp le tse kholo joalo ka 10kb li ka fumanoa.
◮ Ts'ebetso e phahameng ea ho hlaphoheloa: Ts'ebetso ea ho hlaphoheloa hangata e feta 80%, 'me e phahameng ka ho fetisisa e ka fihla ho feta 95%.
◮ Lebelo le potlakileng:Ho bonolo ho sebetsa, ho hlaphoheloa ha sekhechana sa DNA ho ka phethoa nakong ea metsotso e 15 mme primer dimer e ka tlosoa ka kotloloho ntle le ho hlaphoheloa ha gel.
◮ Safety: Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮ Boleng bo phahameng: Likaroloana tse fumanoeng tsa DNA li hloekile haholo, tse ka kopanang le liteko tse fapaneng tse latelang.
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Moemeli oa Phallo ea Mohlala
◮Ho bonolo ho sebetsa:mohlala oa lysis ha o ntse o bokella mohlala
◮E potlakile ebile e bonolo:Ha ho na tlhoko ea ho ntšoa ha nucleic acid, e loketseng ho fumanoa ka tekanyo e kholo
◮Sekoahelo se pharaletseng sa ts'ebeliso:PCR ka ho toba ka mor'a ho bokella sampole
◮E bolokehile ebile ea tšepahala:Fast inactivate kokoana-hloko, netefatsa polokeho ho isa tekanyong e kholo
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RT-PCR Easyᵀᴹ I(Mohato o le Mong)
◮Sesebelisoa sa mohato o le mong se etsa hore ho fetoleloa ka morao le PCR ho etsoe ka tube e le 'ngoe.E hloka feela ho eketsa template ea RNA, li-primer tsa PCR le RNase-Free ddH2O.
◮Tlhahlobo ea nako ea 'nete ea RNA e ka etsoa kapele le ka nepo.
◮Setsi sena se sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
◮Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
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RT-PCR Easyᵀᴹ II(Mehato e Meli)
◮Tlhahlobo ea nako ea 'nete ea RNA e ka etsoa kapele le ka nepo.
◮Setsi sena se sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
◮ Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
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Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits ho tloha Mouse Tail
Thepa e potlakileng ka ho fetisisa lefatšeng ea ho ntša DNA ea mohatla oa mouse, e ka ntšang DNA ea boleng bo holimo mohatleng oa toeba nakong ea hora e le 'ngoe.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Baktheria DNA Isolation Kit Bacterial Genomic DNA Extraction Purification Kits
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa ho libaktheria karolong ea kholo ea logarithmic.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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CCND1/IGH Mebala e Mebeli ea Mebala e Mebeli ea Fusion Probe
Ho latela molao-motheo oa DNA base complementary pairing, CCND1 orange-red probe le IGH green probe li ile tsa sebelisoa ho kopanya ka tatellano ea sepheo sa DNA khubung, 'me tlhahisoleseding ea boemo ba lefutso khubung e ile ea hlokomeloa le ho hlahlojoa ka tlas'a microscope ea fluorescence.
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RB1/1q21 Probe ea Mebala e Mebeli
Fluorescence in situ hybridization (FISH) e ipapisitse le molao-motheo oa ho kopanya li-bases tsa DNA, le pono ea mats'oao a hybridization a DNA e ngotsoeng ka fluorescence ka tatelano ea eona ea sepheo sa DNA ka har'a nucleus ea sele tlas'a microscope ea fluorescence.
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HER2/CSP17 Probe ea Mebala e Mebeli
Fluorescence in situ hybridization (FISH) e ipapisitse le molao-motheo oa ho kopanya li-bases tsa DNA, le pono ea mats'oao a hybridization a DNA e ngotsoeng ka fluorescence ka tatelano ea eona ea sepheo sa DNA ka har'a nucleus ea sele tlas'a microscope ea fluorescence.
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ETV6 Mebala e Hlahang ea Melato e Menyenyane Probe
Ho latela DNA base complementary pairing principle, ETV6 orange-red probe le ETV6 green probe li ile tsa sebelisoa ho kopanya ka tatellano ea sepheo sa DNA khubung, 'me tlhahisoleseding ea boemo ba liphatsa tsa lefutso khubung e ile ea hlokomeloa le ho hlahlojoa ka tlas'a microscope ea fluorescence.
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