Serum(Plasma) miRNA Isolation Kit
Tlhaloso ea Kit:
◆Sete e felletseng ea RNase-Free, ha ho na lebaka la ho tšoenyeha ka ho senyeha ha RNA.
◆DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
◆Tlhahiso e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
◆Lebelo le potlakileng: Ts'ebetso e bonolo ebile e ka phethoa nakong ea metsotso e 30.
◆Polokeho: Ha ho hlokahale ho ntšoa ha reagent ea organic.
◆Boleng bo phahameng: Likaroloana tse nkiloeng tsa RNA li na le bohloeki bo phahameng, ha li na protheine le litšila tse ling, 'me li ka kopana le likopo tse fapaneng tsa liteko tse tlase.
Litlhaloso
Setsi sena se sebelisa "spin column le formula" e ntlafalitsoeng ke k'hamphani ea rona, e ka ntšang likhechana tse nyane tsa miRNA ka tlase ho 100nt ho serum le plasma.The kit e fana ka DNA-Cleaning Column, e ka arolang habonolo supernatant le tissue lysate le adsorb le ho tlosa DNA ka sampuli, e bonolo le e bolokang nako;RNA-feela Column e ka tlama RNA hantle, 'me ka foromo e ikhethang, e ka sebelisoa ka nako e le ngoe.Etsa palo e kholo ea lisampole.
Tsamaiso eohle ke RNase-Free, e le hore miRNA e hloekisitsoeng e se ke ea senyeha;Buffer miBRW1 le Buffer miBRW2 buffer system ea ho hlatsoa li etsa hore RNA e fumanehe ntle le tšilafalo ea protheine, DNA, ion, le organic compound.
Litlhaloso
5T, 50T, 100T, 200T
Likarolo tsa Kit
| Serum / Plasma miRNA Isolation Kit | ||||
| Likahare tsa Kit | RE-0111T | RE-01111 | RE-01112 | RE-01114 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miBRL1* | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRL2* | 1.2ml | 12ml | 24ml | 48ml |
| Buffer miBRW1 | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-Free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| Lekholomo la RNA feela | 5 likotoana | 50likotoana | 100likotoana | 200likotoana |
| DNA-Ho hloekisa Column | 5 likotoana | 50likotoana | 100likotoana | 200likotoana |
| IFU | 1 sengoathoana | 1 sengoathoana | 1 sengoathoana | 1 sengoathoana |
Likarolo le melemo
◆Sete e felletseng ea RNase-Free, ha ho na lebaka la ho tšoenyeha ka ho senyeha ha RNA.
◆DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
◆Tlhahiso e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
◆Lebelo le potlakileng: Ts'ebetso e bonolo ebile e ka phethoa nakong ea metsotso e 30.
◆Polokeho: Ha ho hlokahale ho ntšoa ha reagent ea organic.
◆Boleng bo phahameng: Likaroloana tse nkiloeng tsa RNA li na le bohloeki bo phahameng, ha li na protheine le litšila tse ling, 'me li ka kopana le likopo tse fapaneng tsa liteko tse tlase.
Litekanyetso tsa kit
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisa miRNA ho tsoa ho serum ea liphoofolo le ea batho le plasma.
Sesebelisoa sa miRNA
MiRNA e hloekisitsoeng ke Serum/Plasma miRNA Isolation Kit e ka sebelisoa litekong tse fapaneng tse tlase tsa limolek'hule, tse kang: RT-PCR, Real Time RT-PCR, Northern Blot, chip analysis, joalo-joalo.
Phallo ea mosebetsi
Maemo a polokelo
Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore(15-25°C).Ihaeba e hloka ho bolokoa nako e telele,Ka kopoe bolokiloe ka mocheso oa 2-8 ° C.
