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RT-PCR Easyᵀᴹ I(Mohato o le Mong)
◮Sesebelisoa sa mohato o le mong se etsa hore ho fetoleloa ka morao le PCR ho etsoe ka tube e le 'ngoe.E hloka feela ho eketsa template ea RNA, li-primer tsa PCR le RNase-Free ddH2O.
◮Tlhahlobo ea nako ea 'nete ea RNA e ka etsoa kapele le ka nepo.
◮Setsi sena se sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
◮Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
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Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits ho tloha Mouse Tail
Thepa e potlakileng ka ho fetisisa lefatšeng ea ho ntša DNA ea mohatla oa mouse, e ka ntšang DNA ea boleng bo holimo mohatleng oa toeba nakong ea hora e le 'ngoe.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Baktheria DNA Isolation Kit Bacterial Genomic DNA Extraction Purification Kits
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa ho libaktheria karolong ea kholo ea logarithmic.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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ATM/CSP11 Dual Color Probe
Ho ea ka molao-motheo oa ho kopanya li-bases tsa DNA, lipatlisiso tsa ATM orange le CSP11 green probe li ile tsa sebelisoa ho kopanya ka tatellano ea DNA khubung, 'me tlhahisoleseding ea boemo ba lefutso khubung e ile ea hlokomeloa le ho hlahlojoa ka tlas'a microscope ea fluorescence.
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ERG1/TERT Probe ea Mebala e Mebeli
Fluorescence in situ hybridization (FISH) e ipapisitse le molao-motheo oa ho kopanya li-bases tsa DNA, le pono ea mats'oao a hybridization a DNA e ngotsoeng ka fluorescence ka tatelano ea eona ea sepheo sa DNA ka har'a nucleus ea sele tlas'a microscope ea fluorescence.
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DNA/RNA Extraction Kit (Lifaha tsa Magnetic)
- Ts'ebetso eohle ea ho itšehla thajana e bolokehile ebile e bonolo
- DNA e ntšitsoeng e sa lefelloeng e na le chai e ngata, bohloeki bo phahameng le boleng bo ka tšeptjoang
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20q12/20q13.33 Probe ea Mebala e Mebeli
Ho ea ka molao-motheo oa ho kopanya li-bases tsa DNA, 20q12 (D20S108) probe ea orange le 20q33.3 e tala li ile tsa sebelisoa ho kopanya ka tatellano ea DNA khubung, 'me tlhahisoleseding ea boemo ba lefutso khubung e ile ea hlokomeloa le ho hlahlojoa ka tlas'a maekorosekopo ea fluorescence.
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p53/D13S319 Probe ea Mebala e Mebeli
Fluorescence in situ hybridization (FISH) e ipapisitse le molao-motheo oa ho kopanya li-bases tsa DNA, le pono ea mats'oao a hybridization a DNA e ngotsoeng ka fluorescence ka tatelano ea eona ea sepheo sa DNA ka har'a nucleus ea sele tlas'a microscope ea fluorescence.
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EndoFree Maxi Plasmid Kit (Spin Column)
Ts'ebetso eohle ea ho ntša e nka hora e le 'ngoe feela, ho netefatsa ts'ebetso e bonolo le e potlakileng.
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MALT1 Dual Color Break Apart probe
Ho latela molao-motheo oa DNA base complementary pairing, MALT1 orange-red probe le MALT1 green probe li ile tsa sebelisoa ho kopanya ka tatellano ea sepheo sa DNA khubung, 'me tlhahisoleseding ea boemo ba liphatsa tsa lefutso khubung e ile ea hlokomeloa le ho hlahlojoa ka tlas'a microscope ea fluorescence.
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D7S522/CSP7 Probe ea Mebala e Mebeli
Fluorescence in situ hybridization (FISH) e ipapisitse le molao-motheo oa ho kopanya li-bases tsa DNA, le pono ea mats'oao a hybridization a DNA e ngotsoeng ka fluorescence ka tatelano ea eona ea sepheo sa DNA ka har'a nucleus ea sele tlas'a microscope ea fluorescence.
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DAPI Counterstain
Karolo e ka sehloohong ea tharollo ea ho tsosolosa DAPI ke 4-phenylindole, e ka kenang lera la sele 'me e tlama ka matla ho DNA.
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