Letoto le tobileng la RT-qPCR
PCR e tobileng
Direct PCR ke eng
Direct PCR ke mokhoa oa ho holisa DNA ka kotloloho ho tsoa maling, lisele tsa liphoofolo, mohatla oa likhoto, sele, tsebe ea rat, tlhapi ea liqoaha, mahe a tlhapi, makhasi a semela, lipeo kapa sampole e 'ngoe ea tlhaho ea tlhaho ea tlhaho ntle le ho etsa mehato ea ho itšehla thajana le ho itlhoekisa.Mokhoa oa Direct PCR o ka fokotsa nako ea liteko haholo, le litšenyehelo tsa merero ea genotyping le ea bongata bo bongata.E boetse e fana ka khetho e betere ha u tobane le mathata a ho holisa sampole e nyane haholo moo mohato oa tlhoekiso o ka lebisang ho tahlehelo ea sampole.
FOREGENE's PCR Mix e na le khanyetso e matla ea khatello ea maikutlo, ka lebaka la FOREGENE matla a patented Taq enzyme( tumello ea molao: ZL20161034512.0 (Chaena), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)Enzyme ea eona ea Taq e sebelisa thekenoloji ea DNA recombination ho kopanya hape sebaka sa sebopeho se tlamang sa DNA sa archaeal nucleic acid e tlamang protheine e nang le Taq DNA polymerase e thibelang mocheso ho etsa template ea DNA e nang le kamano e eketsehileng.Taq e ntlafetseng e chesang e bolokang mesebetsi e sa tšoaneng ea DNA polymerase ea pele. Enzyme ena ea Taq e ka tlama ho template ea DNA le ho qala motsoako oa DNA ka li-template tse tlaase tsa mahloriso le libaka tse rarahaneng.
Mohlala, ho bonolo joalo ka ho kenya sele ea hau, semela (lekhasi le lenyane, motso kapa peo) kapa sampole ea phoofolo ho buffer, le ho eketsa motsoako o ikhethang oa li-primers ka har'a li-tubes tsa PCR le ho o tsamaisa ka sebaesekele se futhumatsang.Ho sebetsa hantle ha lisebelisoa ho loketse tlhahlobo e kholo ea sampole moo ho boloka nako ho leng bohlokoa haholo.
Sistimi ea karabelo ea PCR e nang le enzyme ea UNG le dUTP e khethoa ho latela litlhoko tsa liteko, tse ka qobang tšilafalo e bakiloeng ke lihlahisoa tsa amplification tsa PCR.
Melemo eaDPCR e nepahetseng
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| PCR e tobileng | Mokhoa oa setso (RNA purification+ Coventional PCR) |
| Tšebeliso ea lintho tse bonahalang | Tšebeliso ea lisele | Nyane | Haholo |
| Ho lokisetsa lithempleite | Mofuta oa thepa | Ha ho hlokahale ho sila | Mehlala ea ho sila |
| Tshebetso | Mofuta oa tube e le 'ngoe, 10min | ts'ebetso e rarahaneng, 1h | |
| Flux | 1-96 | 1-24 | |
| Boitšoaro ba PCR | mokhoa oa ho arabela | Motsoako o ntlafalitsoeng oa 2 × PCR | Dntp, MgCl2,10×PCR Buffer,Taq enzyme, |
Akopo
01 Ho tsebahatsa likokoana-hloko tsa pathogenic
02 Boitsebiso bo fetotsoeng ka liphatsa tsa lefutso, genotyping
03 Multiplex PCR / SNP ho lemoha / PCR-RFLP
04 Tatelano/ ho kopanya
Letoto la Lihlahisoa
| Letoto | Lebitso la Sehlahiswa | Litlhaloso | Nomoro ea lethathamo | Maemo a polokelo |
| Letoto le tobileng la RT-qPCR | 200T | DRT-01011 | Karolo ea I 4℃，Karolo II -20℃ | |
| 1000T | DRT-01012 |
| Letoto | Lebitso la Sehlahiswa | Litlhaloso | Nomoro ea lethathamo | Maemo a polokelo |
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Mali Direct PCR Series | 200 × 20μl rxns | TP-04111 | -20 ℃ | |
| 2000 × 20μl rxns | TP-04113 | |||
| 200 × 20μl rxns | TP-04121 | -20 ℃ | ||
| 2000 × 20μl rxns | TP-04123 | |||
| 200 × 20μl rxns | TP-04211 | -20 ℃ | ||
| 2000 × 20μl rxns | TP-04213 | |||
| 200 × 20μl rxns | TP-04221 | -20 ℃ | ||
| 2000 × 20μl rxns | TP-04223 |
| Series | Lebitso la Sehlahiswa | Litlhaloso | Nomoro ea lethathamo | Maemo a polokelo |
| Phoofolo Direct PCR Series | Animal Tissue Direct PCR Kit(65℃ lysis) | 200 × 20μl rxns | TP-01111 | Karolo ea I 4℃，Karolo II -20℃ |
| 2000 × 20μl rxns | TP-01113 | |||
| Animal Tissue Direct PCR Kit(65℃ lysis) -UNG | 200 × 20μl rxns | TP-01121 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-01123 | |||
| Mohatla oa Mouse SuperDirectTMPCR Kit (thempereichara ea mocheso oa kamore) | 200 × 20μl rxns | TP-01311 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-01313 | |||
| Mouse Tail Direct PCR Kit(65℃ lysis) | 200 × 20μl rxns | TP-01331 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-01333 | |||
| Mouse Tail Direct PCR Kit(65℃ lysis) - UNG | 200 × 20μl rxns | TP-01341 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-01343 | |||
| Zebra Fish Direct PCR Kit(65℃ lysis) | 200 × 20μl rxns | TP-01411 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-01413 | |||
| Zebra Fish Direct PCR Kit(65℃ lysis) - UNG | 200 × 20μl rxns | TP-01421 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-01423 |
| Letoto | Lebitso la Sehlahiswa | Litlhaloso | Nomoro ea lethathamo | Maemo a polokelo |
| Semela Direct PCR Series | 200 × 20μl rxns | TP-02111 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-02113 | |||
| 200 × 20μl rxns | TP-02121 | Karolo ea I 4℃，Karolo II -20℃ | ||
| 2000 × 20μl rxns | TP-02123 | |||
| Plant Leaf Direct PCR Plus Kit | 200 × 20μl rxns | TP-02131 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-02133 | |||
| Plant Leaf Direct PCR Plus Kit -UNG | 200 × 20μl rxns | TP-02141 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-02143 | |||
| 200 × 20μl rxns | TP-03111 | Karolo ea I 4℃，Karolo II -20℃ | ||
| 2000 × 20μl rxns | TP-03113 | |||
| 200 × 20μl rxns | TP-03121 | Karolo ea I 4℃，Karolo II -20℃ | ||
| 2000 × 20μl rxns | TP-03123 | |||
| 200 × 20μl rxns | TP-03131 | Karolo ea I 4℃，Karolo II -20℃ | ||
| 2000 × 20μl rxns | TP-03133 | |||
| 200 × 20μl rxns | TP-03141 | Karolo ea I 4℃，Karolo II -20℃ | ||
| 2000 × 20μl rxns | TP-03143 | |||
| Plant Seed Direct PCR Plus Kit I(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03151 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-03153 | |||
| Plant Seed Direct PCR Plus Kit I - UNG(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03161 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-03163 | |||
| 200 × 20μl rxns | TP-03171 | Karolo ea I 4℃，Karolo II -20℃ | ||
| 2000 × 20μl rxns | TP-03173 | |||
| Plant Seed Direct PCR Plus Kit II - UNG(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03181 | Karolo ea I 4℃，Karolo II -20℃ | |
| 2000 × 20μl rxns | TP-03183 |
