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Mali a DNA Mini Kit
Ka potlako hloekisa DNA ea boleng bo phahameng ba genomic maling a anticoagulated (<1ml).
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Kakaretso ea Plasmid Mini Kit Kakaretso ea Plasmid DNA Extraction Miniprep Mini Kits
Ka potlako hloekisa plasmid DNA ea boleng bo holimo ho tsoa ho libaktheria tse fetotsoeng bakeng sa liteko tse tloaelehileng tsa baeloji ea limolek'hule joalo ka phetoho le tšilo ea enzyme.
◮Tlosa RNA ntle le ho eketsa RNase
◮E loketseng- centrifugation e etsoa ka mocheso oa kamore
◮Ka potlako- ts'ebetso e ka phethoa ka metsotso e 20
◮Sireletsehile- ha ho na reagent ea tlhaho e sebelisoang
◮Bohloeki bo phahameng—OD260/280≈1.7-1.9
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RT Easyᵀᴹ I Master Premix bakeng sa motsoako oa pele oa cDNA
- Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 25 feela ho phethela motsoako oa cDNA ea pele.
- Sistimi e phahameng ea sensitivity reverse transcript, litempele tsa boemo ba pg le tsona li ka fumana cDNA ea boleng bo holimo.
- Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso oa karabelo ea sistimi e ka ba holimo ho 50 ℃,'me e na le ts'ebetso e ntle ea ho fetolela ka morao.
- Esita le disampole tse sa hloekang tsa RNA (joala bo fihlang ho 45%, letsoai la guanidine ho fihla ho 750mM) li ka ba tlas'a karabelo e fapaneng ea transcript.
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RT Easy II(e nang le gDNase) Master Premix bakeng sa motsoako oa pele oa cDNA bakeng sa Real Time PCR e nang le gDNase
-Bokhoni bo botle ba ho tlosa gDNA, e ka tlosa gDNA ka template ka hare ho metsotso ea 2.
-Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 15 feela ho phethela motsoako oa cDNA ea pele.
Li-templates tse rarahaneng: litempele tse nang le litaba tse phahameng tsa GC le sebopeho sa bobeli se rarahaneng le tsona li ka khutlisoa ka katleho e phahameng.
-High-sensitivity reverse transcription system, litempele tsa pg-level le tsona li ka fumana cDNA ea boleng bo holimo.
-Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso o nepahetseng oa karabelo ke 42℃, 'me e ntse e na le ts'ebetso e ntle ea ho fetolela ho 50℃.
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Nako ea 'Nete PCR Easyᵀᴹ-SYBR Green I kit
E bonolo—2X PCR Mix ho fokotsa phoso ea liteko le nako ea ts'ebetso
Specific-optimized buffer le hot-start Taq enzyme e ka thibela ho holisoa ho sa hlakang le ho thehoa ha primer dimer.
Kutloelo-bohloko e phahameng - e khona ho bona likopi tse tlase tsa template
E sebetsa ka mokhoa o fapaneng - e tsamaellana le lisebelisoa tse ngata tsa nako ea nnete tsa PCR
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Liphoofolo Kakaretso ea RNA Isolation Kit Kakaretso ea RNA ea ho Ntša le ho Hloekisa Lithako tsa Liphoofolo le Lisele
Ka potlako le ka mokhoa o atlehileng o ntša RNA ea boleng bo holimo le ea boleng bo holimo ho tsoa liseleng tse fapaneng tsa liphoofolo.
Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.Tsamaiso eohle ke RNase-Free
Tlosa DNA ka katleho u sebelisa DNA-Cleaning Column
Tlosa DNA ntle le ho eketsa DNase
E bonolo - ts'ebetso eohle e phethoa ka mocheso oa kamore
Ka potlako-ts'ebetso e ka phethoa ka metsotso e 30
E bolokehile - ha ho na reagent ea tlhaho e sebelisoang
Bohloeki bo phahameng-OD260/280≈1.8-2.1
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Cell Total RNA Isolation Kit Total RNA Isolation Purification Kits ho tloha seleng
RNA e hloekisitsoeng haholo le ea boleng bo holimo e ka fumanoa liseleng tse fapaneng tsa setso ka metsotso e 11.
RNase-Mahala
E sebetsa ka mocheso oa kamore (15-25 ℃)
Ka DNA-Cleaning Column
Kotulo e phahameng ea RNA
Ka potlako: Qetella ho ntša ka mor'a metsotso e 11
Polokeho: Ha ho na Organic Chemical
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Mali a DNA Midi Kit (1-5ml) Mali a Isolation Midi Kits for DNA from Blood
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa maling a anticoagulated (1-5ml).
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Buccal Swab/FTA Card DNA Isolation Kit Genomic DNA Extraction or Purifiction Kit from Buccal Swabs
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa lisampoleng tsa buccal swab/FTA Card.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Foregene DNA Identification System 27Y
Foregene DNA Identification System 27Y e sebelisa thekenoloji ea ho ngola ka mebala e tšeletseng ea fluorescent ho holisa 27 Y-STR loci ka nako e le 'ngoe.Ho kenyeletsoa: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS39, DY398, DY398 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Kit e na le bokhoni bo botle ba ho thibela ho thibela, 'me e ka ntlafatsa ka ho toba pampiri ea ho hloekisa, karete ea FTA, swab ea k'hothone, karete ea saliva, oral swab,' me e ka boela ea sebelisoa ho ntša le ho hloekisa litempele tsa DNA ho tloha thepa ea linyeoe.
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Foregene DNA Identification System 38Y-C (Nyee)
Foregene Sistimi ea ho tsebahatsa DNA 38Y-C e sebelisa theknoloji ea mebala e tšeletseng ea ho ngola fluorescent ho holisa 38 Y chromosome STR sebaka, 3 Y-Indel lociletse peli tse ka hare ho laola boleng loci ka nako e le 'ngoebakeng sa ho holisa Mehlala ea linyeoe tse ntšitsoeng ho lithempleite tsa DNA tse hloekisitsoeng.
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Foregene DNA Identification System 30A (Non-Extraction)
FOREGENE DNA identification system 30A e sebelisa theknoloji ea mebala e tšeletseng ea ho ngola fluorescent hoholisa 29autosomalSSebaka sa TR, ngoeLocus ea Amel le sebaka se le seng sa Yindel ka nako e le 'ngoe,' me e ka holisa pampiri ea sefa ka kotloloho, FTAlikarete, li-swabs tsa k'hothone, likarete tsa mathe,le mswab ea kantle.
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