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Ho bonolo RNase Inhibitor
◮RNase inhibitor e ncha e entsoeng ke mouse e hlahisoang ke libaktheria tsa E. coli tse sebelisang theknoloji ea ho kopanya liphatsa tsa lefutso.Inhibitor e thibela ts'ebetso ea RNase ka ho se kopane le RNase 1: 1 ntle le tlholisano, kahoo e sireletsa.RNAbotšepehi , 'me e ka thibela ka katlehotheRNase A, B, C mosebetsi , empa eseng RNase T1, T2, H, joalo-joalo; E khutlisetsoa morao ea tlamo ea RNase Inhibitor le RNase , 'me e rarahaneng e ka aroloa ke urea le sulfhydryl reagents, e le hore RNase renatures le inhibitor e ke ke ea hlola e e-ba teng.
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Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)
Tšebeliso e tobileng ea li-zebrafish lysate e le template bakeng sa ho hōlisa PCR, ha ho na ho ntšoa ka thōko ho DNA, ka potlako le ka kutloisiso e phahameng.E kenyellelitse UNG tsamaiso e khahlanong le tšilafalo ho felisa lintho tse fosahetseng.
◮Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.
◮Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.
◮Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, 'me ho buuoa ho bonolo.
◮Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
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Taq DNA Polymerase e bonolo
◮Boemo bo phahameng: Enzyme e na le ts'ebetso e itseng ea ho qala ho chesa.
◮Phatlalatso e potlakileng: 10 sec/kb.
◮Template e feto-fetohang haholo: e ka sebelisoa ho holisa boleng bo holimo ba GC, tse fapaneng tse thata ho holisa template ea DNA.
◮Botšepehi bo matla: Taq Enzyme e tloaelehileng makhetlo a 6.
◮Botsitso bo matla ba mocheso: E ka behoa ho 37 ° C ka beke 'me e boloka ts'ebetso e fetang 90%.
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HS Taq DNA Polymerase
◮Boemo bo phahameng: Enzyme e nang le ts'ebetso e phahameng ea ho qala ho chesa.
◮Phatlalatso e potlakileng: 10 sec/kb.
◮Boemo bo phahameng ba template : e ka sebelisoa ho holisa Phahameng ka nepoGCbolenglemefuta e fapaneng e thata ho holisa template ea DNA.
◮Botšepehi bo matla: Botšepehi ke makhetlo a 6of E tloaelehileng ea Taq Enzyme.
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Animal Tissue Direct PCR kit (ntle le Sampling Tools) Protocol
Sebelisa lysate ea linama tsa liphoofolo ka kotloloho joalo ka template ea ho holisa PCR, ntle le ho ntšoa ka thoko ho DNA, kutlo e potlakileng le e phahameng.
◮Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.
◮Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.
◮Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, 'me ho buuoa ho bonolo.
◮Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
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Nako ea 'Nete PCR Easyᵀᴹ-Taqman
◮E bonolo-2 × PCR Mix ho fokotsa phoso ea liteko le nako ea ts'ebetso
◮Specific-optimized buffer le hot-start Taq enzyme e ka thibela ho holisoa ho sa hlakang le ho thehoa ha primer dimer.
◮Kutloelo-bohloko e phahameng - e khona ho bona likopi tse tlase tsa template
◮E sebetsa ka mokhoa o fapaneng - e tsamaellana le lisebelisoa tse ngata tsa nako ea nnete tsa PCR
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RT Easyᵀᴹ II Master Premix bakeng sa motsoako oa pele oa cDNA bakeng sa nako ea 'nete ea PCR
- Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 20 feela ho phethela motsoako oa cDNA ea pele.
- Sistimi e phahameng ea sensitivity reverse transcript, litempele tsa boemo ba pg le tsona li ka fumana cDNA ea boleng bo holimo.
- Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso oa karabelo ea sistimi e ka ba holimo ho 50 ℃, 'me e na le ts'ebetso e ntle ea ho fetolela ka morao.
- Esita le disampole tse sa hloekang tsa RNA (joala bo fihlang ho 60%, letsoai la guanidine ho fihla ho 750mM) li ka ba tlas'a karabelo e fapaneng ea transcript.
- 2× RT KAPA-E bonoloTM Mix e kentse li-primers tse ntlafalitsoeng tsa khatiso (Random Primer, Oligo(dT)18 Sethome).
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Blood SuperDirectᵀᴹ PCR Kit-EDTA Blood Direct PCR Master Mix bakeng sa Genotyping of Blood
Sebelisa ka kotloloho mali a EDTA a anticoagulated e le template ea ho holisa PCR, e nang le kutlo e phahameng le matla a ho holisa.
◮Ha ho na tlhoko ea tlhoekiso ea DNA e jang nako e ngata le e theko e boima, ha ho na kalafo, le tšebeliso e tobileng ea mali a felletseng joalo ka template ea PCR.
◮Sistimi ena e na le matla a ho holisa 'me e khona ho bona ka hloko liphatsa tsa lefutso tsa DNA le likhechana tsa DNA maling.
◮2 × SuperEasyTMMix-EDTA Mamello e phahameng ea mali: ho fihla ho 45% maling a motho le 20% maling a toeba.
◮Mohlala o koaletsoe ka botlalo, kahoo ha ho na lebaka la ho tšoenyeha ka tšilafalo ea sampole le liphetho tse fosahetseng tsa PCR.
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PCR Heroᵀᴹ (Ka dae)
◮Botšepehi bo phahameng: makhetlo a 6 ho feta a enzyme e tloaelehileng ea Taq;
◮Lebelo la amplification le potlakileng
◮Ho ikamahanya le maemo ho feta template
◮Bokhoni bo phahameng ba amplification
◮Ho mamella tikoloho ho matla: ho behoa ho 37 ° C ka beke, ho boloka mosebetsi o fetang 90%;
◮E na le ts'ebetso ea 5'→ 3' DNA polymerase le 5'→ 3' exonuclease, ntle le ts'ebetso ea 3'→5' ea exonuclease.
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Blood SuperDirectᵀᴹ PCR Kit-Heparin Blood Direct PCR Master Mix bakeng sa Genotyping of Blood
Sebelisa ka kotloloho mali a felletseng a anticoagulated ea heparin joalo ka template ea ho holisa PCR, e nang le kutlo e phahameng le bokhoni bo phahameng ba ho holisa.
◮Ha ho na tlhoko ea tlhoekiso ea DNA e jang nako e ngata le e theko e boima, ha ho na kalafo, le tšebeliso e tobileng ea mali a felletseng joalo ka template ea PCR.
◮Sistimi ena e na le matla a ho holisa 'me e khona ho bona ka hloko liphatsa tsa lefutso tsa DNA le likhechana tsa DNA maling.
◮2 × SuperEasyTMMix-Heparin Mamello e phahameng ea mali: ho fihlela ho 45% maling a motho le 20% maling a litoeba.
◮Mohlala o koaletsoe ka botlalo, kahoo ha ho na lebaka la ho tšoenyeha ka tšilafalo ea sampole le liphetho tse fosahetseng tsa PCR.
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Blood SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Blood Direct PCR Master Mix bakeng sa Genotyping of Blood
Sebelisa ka kotloloho mali a felletseng a anticoagulated ea heparin joalo ka template ea ho holisa PCR, e nang le kutlo e phahameng le bokhoni bo phahameng ba ho holisa.Lenaneo la UNG le khahlanong le tšilafalo le ekelitsoe ho thibela mekhoa e fosahetseng.
◮Sistimi ena e na le matla a ho holisa 'me e khona ho bona ka hloko liphatsa tsa lefutso tsa DNA le likhechana tsa DNA maling.
◮2 × SuperEasyTMMix(UNG) -Heparin Mamello e phahameng ea mali: ho fihla ho 45% maling a motho le 20% maling a toeba.
◮Mohlala o koaletsoe ka botlalo, kahoo ha ho na lebaka la ho tšoenyeha ka tšilafalo ea sampole le liphetho tse fosahetseng tsa PCR.Sistimi e khahlanong le tšilafalo ea PCR
◮2 × SuperEasyTMKopanya (UNG), ka katleho felisa tšilafalo e bakoang ke lihlahisoa tsa PCR, 'me u netefatse ho nepahala le ho nepahala ha amplification.
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Blood SuperDirectᵀᴹ PCR Kit(UNG)-EDTA Blood Direct PCR Master Mix bakeng sa Genotyping of Blood
Sebelisa ka kotloloho mali a EDTA a anticoagulated e le template ea ho holisa PCR, e nang le kutlo e phahameng le matla a ho holisa.Lenaneo la UNG le khahlanong le tšilafalo le ekelitsoe ho thibela mekhoa e fosahetseng.
◮Ha ho na tlhoko ea tlhoekiso ea DNA e jang nako e ngata le e theko e boima, ha ho na kalafo, le tšebeliso e tobileng ea mali a felletseng joalo ka template ea PCR.
◮Sistimi ena e na le matla a ho holisa 'me e khona ho bona ka hloko liphatsa tsa lefutso tsa DNA le likhechana tsa DNA maling.
◮2 × SuperEasyTMMix(UNG) -EDTA e na le mamello e phahameng ea mali: ho fihla ho 45% maling a motho le 20% maling a toeba.
◮Mohlala o koaletsoe ka botlalo, kahoo ha ho na lebaka la ho tšoenyeha ka tšilafalo ea sampole le liphetho tse fosahetseng tsa PCR.Sistimi e khahlanong le tšilafalo ea PCR
◮2 × SuperEasyTMMix(UNG) -EDTA, ka katleho e felisa tšilafalo e bakoang ke lihlahisoa tsa PCR, 'me e netefatsa ho nepahala le ho nepahala ha amplification.
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