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Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Mohatla)(bakeng sa Genotyping)
Ka ho toba sebelisa mouse tail lysate e le template bakeng sa ho hōlisa PCR, ntle le ho ntša DNA e arohaneng, ka potlako le ka kutloisiso e phahameng.
◮Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.
◮Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.
◮Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, 'me ho buuoa ho bonolo.
◮Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
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Animal Tissue Direct PCR kit-UNG (ntle le Sampling Tools) Protocol
Sebelisa lysate ea linama tsa liphoofolo ka kotloloho joalo ka template ea ho holisa PCR, ntle le ho ntšoa ka thoko ho DNA, kutlo e potlakileng le e phahameng.E kenyellelitse UNG tsamaiso e khahlanong le tšilafalo ho felisa lintho tse fosahetseng.
◮Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.
◮Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.
◮Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, 'me ho buuoa ho bonolo.
◮Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
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Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent(Mouse Mohatla)(bakeng sa Genotyping)
Ka ho toba sebelisa mouse tail lysate e le template bakeng sa ho hōlisa PCR, ntle le ho ntša DNA e arohaneng, ka potlako le ka kutloisiso e phahameng.
◮Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.
◮Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.
◮Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, 'me ho buuoa ho bonolo.
◮Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
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Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(bakeng sa Genotyping)
The mouse tail lysate e sebelisoa ka kotloloho e le template ea ho holisa PCR ntle le ho ntšoa ka thoko ho DNA, e potlakileng ebile e na le kutloelo-bohloko haholo.E kenyellelitse UNG tsamaiso e khahlanong le tšilafalo ho felisa lintho tse fosahetseng.
◮Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.
◮Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.
◮Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, 'me ho buuoa ho bonolo.
◮Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
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Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent(zebrafish)
Ka ho toba sebelisa zebrafish lysate e le template bakeng sa ho hōlisa PCR, ntle le ho ntša DNA e arohaneng, ka potlako le ka kutloisiso e phahameng.
◮Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.
◮Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.
◮Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, 'me ho buuoa ho bonolo.
◮Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
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Lekhasi la semela Direct PCR Plus kit (ntle le lisebelisoa tsa sampole) Protocol Direct PCR ho tsoa ho Plant Material.
Ka ho toba sebelisa polysaccharide le polyphenol lekhasi lysate e le template bakeng sa PCR amplification, ntle le ho ntšoa ka thōko ho DNA, ka potlako le ka kutloisiso e phahameng.
◮Ha ho tlhoekiso ea DNA e jang nako le e turang (Nako, mosebetsi le litšenyehelo li bolokiloe!)
◮Boitsebiso bo fokolang
◮E bonolo - sampole e ka ba tlas'a karabelo ea PCR kapa karabelo ea lysis ntle le ho seha kapa ho sila
◮2 X Mix-ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi
◮Tokiso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa ka metsotso e 50
◮High throughput-lysis reaction e ka phethoa ka poleiti ea PCR ea seliba tse 96
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Plant Seed Direct PCR Kit I/II (ntle le Sampling Tools) Protocol Plant Direct PCR Kits
◮Ha ho tlhoekiso ea DNA e jang nako le e turang (Nako, mosebetsi le litšenyehelo li bolokiloe!)
◮Boitsebiso bo fokolang
◮E bonolo - sampole e ka ba tlas'a karabelo ea PCR kapa karabelo ea lysis ntle le ho seha kapa ho sila
◮2 X Mix-ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi
◮Tokiso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa ka metsotso e 50
◮High throughput-lysis reaction e ka phethoa ka poleiti ea PCR ea seliba tse 96
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Lekhasi la semela Direct PCR hammoho le kit-UNG (ntle le lisebelisoa tsa sampole) Protocol Direct PCR ho tsoa ho Plant Material.
Ka ho toba sebelisa polysaccharide le polyphenol lekhasi lysate e le template bakeng sa PCR amplification, ntle le ho ntšoa ka thōko ho DNA, ka potlako le ka kutloisiso e phahameng.Lenaneo la UNG le khahlanong le tšilafalo le ekelitsoe ho thibela mekhoa e fosahetseng.
◮Ha ho tlhoekiso ea DNA e jang nako le e turang (Nako, mosebetsi le litšenyehelo li bolokiloe!)
◮Boitsebiso bo fokolang
◮E bonolo - sampole e ka ba tlas'a karabelo ea PCR kapa karabelo ea lysis ntle le ho seha kapa ho sila
◮2 X Mix-ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi
◮Tokiso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa ka metsotso e 50
◮High throughput-lysis reaction e ka phethoa ka poleiti ea PCR ea seliba tse 96
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RT-qPCR Easyᵀᴹ (Mohato o le Mong)-Taqman
- E 'ngoe-mohato oa mohato o nolofalletsa ho ngoloa ka morao le PCR ho etsoa ka tube e le 'ngoe, ho hlokahala feela ho eketsa template ea RNA, li-primers tse khethehileng tsa PCR le RNase-Free ddH.2O.
- Tlhahlobo ea nako ea 'nete ea palo ea RNA ea vaerase kapa trace RNA e ka etsoa kapele le ka nepo.
- Thepa e sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
- Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
- RT-qPCR e bonoloTM(Mohato o le mong) -Taqman kit e tla le dae ea ka hare ea ROX, e ka sebelisoang ho tlosa semelo sa matšoao le liphoso tsa matšoao lipakeng tsa liliba, tse loketseng basebelisi ba ho qetela ho li sebelisa mefuteng e fapaneng ea lisebelisoa tsa PCR tse ngata.
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RNAlater (Bakeng sa RNA Stabilization) Tharollo ea RNAlater Stabilization
Ho lokisoa ka potlako ha lisele tsa liphoofolo tse sa tsoa nkuoa, ho thibela tšebetso ea RNase, ho sireletsa RNA ho senyeha.
-Li-reagents li sebelisoa ka ho toba, mohato o le mong o teng, 'me RNA e tsitsisoa hang-hang le ho sireletsoa.
-Ts'ebetso ka mocheso oa kamore, e bonolo, e bolokehile ebile e se chefo.
-Ho tlosa khathatso ea leqhoa le omileng kapa sehatsetsing sa mocheso o tlase bakeng sa ho boloka lisele.
-Lithane li ka bolokoa nako e telele ntle le kotsi ea ho senyeha ha RNA, ho netefatsa lintlha tse tšepahalang tsa profil ea liphatsa tsa lefutso.
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Mohale oa PCR
◮Botšepehi bo phahameng: 6linako tsa enzyme e tloaelehileng ea Taq;
◮Ka potlako alebelo la ho ntlafatsa:lebelo la amplification ke 5-10sec/kb, e leng makhetlo a 6-12 a enzyme e tloaelehileng ea Taq;
◮Ho ikamahanya le maemo ho feta template: e ka holisa ka nepo litempele tse fapaneng tse rarahaneng tsa DNA tse nang le litaba tse phahameng tsa GC mme ho thata ho li holisa;
◮E phahamengMatla a ho holisa: palo ea lipotoloho tsa amplification e tlase ho feta ea enzyme e tloaelehileng ea Taq;
◮Smatlafala Mamello ya tikoloho: e behiloe ho 37°C ka beke, e boloka ho feta90%ts'ebetso;
◮E na le ts'ebetso ea 5'→ 3' DNA polymerase le 5'→ 3' exonuclease, ntle le ts'ebetso ea 3'→5' ea exonuclease.
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PCR Easy
Sistimi e ikhethang ea karabelo le ts'ebetso e phahameng ea Taq DNA Polymerase li etsa hore karabelo ea PCR e be le katleho e phahameng ea ho holisa, boits'oaro le kutlo.
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